Elasticity and adhesion of resting and lipopolysaccharide-stimulated macrophages

Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS-stimulated cells (separation force = 37 ± 3 nN) was almost twice as high as tha...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 2006-01, Vol.580 (2), p.450-454
Main Authors: Leporatti, Stefano, Gerth, Anja, Köhler, Guido, Kohlstrunk, Bernd, Hauschildt, Sunna, Donath, Edwin
Format: Article
Language:English
Subjects:
Adhesion
Cell Adhesion - physiology
Colloidal force microscopy
Cytoskeleton - metabolism
Elasticity
Humans
Lipopolysaccharide
Lipopolysaccharides - pharmacology
Macrophage
Macrophages - cytology
Macrophages - drug effects
Macrophages - physiology
Microscopy, Scanning Probe - methods
Stress, Mechanical
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Colloidal Force Microscopy was employed to study the viscoelastic and adhesive properties of macrophages upon stimulation with lipopolysaccharide (LPS). Force vs. distance measurements were performed. The adhesion of LPS-stimulated cells (separation force = 37 ± 3 nN) was almost twice as high as that of resting macrophages (16 ± 1 nN). Upon retraction pulling of membrane tethers was observed. Tether lengths and forces at which rupture take place did not depend on stimulation. The reduced Young’s modulus K, a measure of cytoskeleton elasticity, was three times lower than that of the control. The data show that LPS has profound effects on cytomechanical and adhesion properties of macrophages.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2005.12.037