Quantitative and qualitative analysis of flavonoid markers in Frucus aurantii of different geographical origin by capillary electrophoresis with electrochemical detection

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-01, Vol.830 (2), p.224-230
• Main Authors: Peng, Youyuan, Liu, Fanghua, Ye, Jiannong
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Buffers; Capillary electrophoresis; China; Citrus - chemistry; Electrochemical detection; Electrochemistry - instrumentation; Electrochemistry - methods; Electrodes; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Flavanones - analysis; Flavonoids; Flavonoids - analysis; Frucus aurantii; Fruit - chemistry; Fundamental and applied biological sciences. Psychology; General pharmacology; Hesperidin - analysis; Hydrogen-Ion Concentration; Medical sciences; Pharmacology. Drug treatments; Reproducibility of Results; Synephrine - analysis; Time Factors
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2005.10.043

A capillary electrophoresis with electrochemical detection (CE-ED) method was developed for the simultaneous determination of four major flavonoid markers (synephrine, naringin, hesperidin and naringenin) in Frucus aurantii of different geographical origin. Operated in a wall-jet configuration, a 300 μm diameter carbon disc electrode was used as the working electrode, which exhibits a good response at +0.85 V (versus saturated calomel electrode) for the analytes. Under the optimum conditions, the analytes were baseline separated within 20 min in a 80 mmol/L borax buffer (pH 8.45). The intra-day relative standard deviations (R.S.D.) and inter-day R.S.D.s were based on the analysis of the standard solution on the same day and on the following 6 consecutive days. The intra-day R.S.D.s ranged from 0.8% (naringin) to 3.6% (hesperidin). The inter-day R.S.D.s ranged from 1.2% (hesperidin) to 4.6% (naringenin). Calibration curves were linear in ranges between 0.05 and 1000 μg/mL for the markers. Limits of detection ranged from a low of 1 × 10 −8 g/mL (hesperidin) to a high of 5 × 10 −7 g/mL (naringin). The method was successfully used in the analysis of F. aurantii of different geographical origin with relatively simple extraction procedures, and the assay results were satisfactory.