Human milk antibodies with polysaccharide kinase activity

• Published in: Immunology Letters 2006-02, Vol.103 (1), p.58-67
• Main Authors: Karataeva, Natal’ya A., Gorbunov, Dmitry, Prokudin, Ivan V., Buneva, Valentina N., Kulminskaya, Anna A., Neustroev, Kirill N., Nevinsky, Georgy A.
• Format: Article
• Language: English
• Subjects: Antibodies, Catalytic - chemistry; Antibodies, Catalytic - metabolism; Antibodies, Catalytic - physiology; Catalysis; Catalytic sIgA; Human milk; Humans; Immunoglobulin A, Secretory - chemistry; Immunoglobulin A, Secretory - metabolism; Immunoglobulin A, Secretory - pharmacology; Milk, Human - chemistry; Milk, Human - enzymology; Milk, Human - immunology; Phosphorylation; Phosphorylation of polysaccharides; Phosphotransferases - chemistry; Phosphotransferases - metabolism; Polysaccharides - chemistry; Polysaccharides - metabolism
• ISSN: 0165-2478; 1879-0542; 1365-2567
• DOI: 10.1016/j.imlet.2005.10.009

It was shown for the first time that a small fraction of milk secretory IgA (sIgA) is tightly bound to oligosaccharides (oligoSACs) and polysaccharides (polySACs). The ability of sIgA to phosphorylate oligo- and polysaccharides was shown to be an intrinsic property of this antibody. In contrast to known kinases, sIgAs with polysaccharide kinase activity can transfer phosphoryl group to oligo- and polysaccharides not only from [γ- 32P]ATP but can also use [ 32P] orthophosphate as a substrate of phosphorylation reaction. An extremely unusual property of polysaccharide kinase Abs is their high affinity for orthophosphate ( K m = 15–77 μM), and orthophosphate is a better substrate than ATP. Two first examples of natural abzymes (Abzs) with synthetic activity were milk sIgA with protein and lipid kinase activities. Polysaccharide kinase sIgA of human milk is the third example of natural antibodies (Abs) with synthetic activity.