Influence of the physiologically widespread substances on the oxidative activity of copper(II) complexes with sinefungin, a nucleoside antibiotic

The oxidation-promoting reactivity of the Cu(II)–sinefungin complex in the presence of hydrogen peroxide was studied at pH 7.4, using N, N-dimethyl- p-nitrosoaniline (NDMA), as well as plasmid DNA as target molecules. Mixture of the complex with H 2O 2 was found to be an efficient oxidant, bleaching...

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Published in:Journal of inorganic biochemistry 2006-02, Vol.100 (2), p.178-185
Main Authors: Mucha, Ariel, Cappanelli, Massimo, Szczepanik, Wojciech, Kaczmarek, Piotr, Skała, Jacek, Jeżowska-Bojczuk, Małgorzata
Format: Article
Language:English
Subjects:
Adenosine - analogs & derivatives
Adenosine - chemistry
Anti-Bacterial Agents - chemistry
Anti-Bacterial Agents - pharmacology
Copper - chemistry
Copper(II) complexes
DNA - drug effects
DNA - metabolism
DNA Damage
Hydrogen-Ion Concentration
Hydroxyl radical
Molecular Structure
Nucleoside antibiotic
Organometallic Compounds - chemistry
Oxidation-Reduction
Plasmid DNA damage
Reactive Oxygen Species - metabolism
Sinefungin
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Summary:The oxidation-promoting reactivity of the Cu(II)–sinefungin complex in the presence of hydrogen peroxide was studied at pH 7.4, using N, N-dimethyl- p-nitrosoaniline (NDMA), as well as plasmid DNA as target molecules. Mixture of the complex with H 2O 2 was found to be an efficient oxidant, bleaching NDMA solution, and generating single- and double-strand breaks in DNA. The oxidative DNA damage was investigated also in the presence of varying amounts of glutathione, histidine, Gly-Gly-His peptide, H2A histone, and ascorbic acid, showing diverse influence of those substances on the cleavage extension.
ISSN:0162-0134
1873-3344
DOI:10.1016/j.jinorgbio.2005.11.001