Effect of repeated restraint stress on the levels of intestinal IgA in mice

• Published in: Psychoneuroendocrinology 2007-07, Vol.32 (6), p.681-692
• Main Authors: Jarillo-Luna, Adriana, Rivera-Aguilar, Víctor, Garfias, Humberto Reyna, Lara-Padilla, Eleazar, Kormanovsky, Alexander, Campos-Rodríguez, Rafael
• Format: Article
• Language: English
• Subjects: Adrenalectomy; Animals; Behavioral psychophysiology; Biological and medical sciences; Catecholamines; Corticosterone - blood; Dexamethasone - pharmacology; Endocrinology & Metabolism; Epinephrine - pharmacology; Fundamental and applied biological sciences. Psychology; Glucocorticoids; Habituation, Psychophysiologic - physiology; Hormones and behavior; IgA; Immunoglobulin A - analysis; Immunoglobulin A - metabolism; Intestine; Intestines - chemistry; Intestines - drug effects; Intestines - immunology; Male; Mice; Mice, Inbred BALB C; Mifepristone - pharmacology; Mucous Membrane - cytology; Mucous Membrane - drug effects; Mucous Membrane - metabolism; Norepinephrine - blood; Plasma cells; Psychiatry; Psychology. Psychoanalysis. Psychiatry; Psychology. Psychophysiology; Receptors, Glucocorticoid - antagonists & inhibitors; Restraint, Physical - physiology; Stress; Stress, Psychological - blood; Stress, Psychological - immunology; Sympathectomy, Chemical
• ISSN: 0306-4530; 1873-3360
• DOI: 10.1016/j.psyneuen.2007.04.009

Summary The effects of restraint stress on the intestinal humoral immune system, particularly those about intestinal IgA production, have not been explored in detail. Thus, the purpose of this study was to assess the effect of restraint stress on the production and secretion of intestinal IgA as well as on the number of IgA+ cells in the intestinal lamina propria. The involvement of glucocorticoids and catecholamines were also evaluated. Mice were exposed to 1 or 4 h restraint stress for 4 d. The intestinal IgA concentration was quantified by ELISA and the number of IgA containing cells in the lamina propria was determined by immunohistochemistry. The effects of restraint were also analyzed in mice submitted to different procedures: adrenalectomy, chemical sympathectomy, treatment with a glucocorticoid antagonist (RU486), dexamethasone and epinephrine. The main findings were that (1) chronic restraint-stress reduced the intestinal IgA concentration without changing the number of IgA+ cells in lamina propria; (2) adrenalectomy restored the production of IgA in stressed mice; (3) RU486 and chemical sympathectomy partially blocked the decrease in intestinal IgA in stressed mice; and (4) pharmacological doses of dexamethasone and epinephrine significantly reduced the intestinal IgA concentration and the number of IgA+ cells. The restraint stress probably reduced the intestinal IgA concentration through the effects of glucocorticoids and catecholamines.