Bronchial Inflammation and Airway Responses to Deep Inspiration in Asthma and Chronic Obstructive Pulmonary Disease

• Published in: American journal of respiratory and critical care medicine 2007-07, Vol.176 (2), p.121-128
• Main Authors: Slats, Annelies M, Janssen, Kirsten, van Schadewijk, Annemarie, van der Plas, Dirk T, Schot, Robert, van den Aardweg, Joost G, de Jongste, Johan C, Hiemstra, Pieter S, Mauad, Thais, Rabe, Klaus F, Sterk, Peter J
• Format: Article
• Language: English
• Subjects: Adult; Aged; Airway Resistance - physiology; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Asthma; Asthma - pathology; Asthma - physiopathology; Biological and medical sciences; Biopsy; Bronchi - pathology; Bronchi - physiopathology; Bronchial Provocation Tests; Bronchoscopy; Case-Control Studies; Chronic obstructive pulmonary disease; Chronic obstructive pulmonary disease, asthma; Cross-Sectional Studies; Female; Humans; Inflammation; Inhalation - physiology; Intensive care medicine; Male; Medical sciences; Middle Aged; Muscle, Smooth - pathology; Muscle, Smooth - physiopathology; Pneumology; Pulmonary Disease, Chronic Obstructive - pathology; Pulmonary Disease, Chronic Obstructive - physiopathology; Pulmonary hypertension. Acute cor pulmonale. Pulmonary embolism. Pulmonary vascular diseases; Respiratory Mucosa - pathology; Respiratory Mucosa - physiopathology; Respiratory system; Smooth muscle; Spirometry
• ISSN: 1073-449X; 1535-4970
• DOI: 10.1164/rccm.200612-1814OC

Deep inspirations provide physiologic protection against airway narrowing in healthy subjects, which is impaired in asthma and chronic obstructive pulmonary disease (COPD). Airway inflammation has been suggested to alter airway mechanics during deep inspiration. We tested the hypothesis that the number of bronchial inflammatory cells is related to deep inspiration-induced bronchodilation in asthma and COPD. In a cross-sectional study, three modified methacholine challenges were performed in 13 patients with mild, persistent asthma, 12 patients with mild to moderate COPD, and 12 healthy control subjects. After a 20-minute period of deep inspiration avoidance, inhalation of methacholine was followed by either one or five deep inspirations, or preceded by five deep inspirations. The response to deep inspiration was measured by forced oscillation technique. Inflammatory cells were counted within the lamina propria and airway smooth muscle area in bronchial biopsies of patients with asthma and COPD. The reduction in expiratory resistance by one and five deep inspirations was significantly less in asthma (mean change+/-SD: -0.5+/-0.8 and -0.9+/-1.0 cm H2O/L/s, respectively) and COPD (+0.2+/-1.1 and +0.4+/-1.0 cm H2O/L/s, respectively) as compared with healthy subjects (-1.5+/-1.3 and -2.0+/-1.2 cm H2O/L/s, respectively; p=0.05 and p=0.001, respectively). In asthma, this was related to an increase in mast cell numbers within the airway smooth muscle area (r=0.73; p=0.03), and in CD4+ lymphocytes in the lamina propria (r=0.61; p=0.04). Inflammation in the airway smooth muscle bundles and submucosa of bronchial biopsies is positively associated with impaired airway mechanics during deep inspiration in asthma, but not in COPD. Clinical trial registered with www.clinicaltrials.gov (NCT OO279136).