Affinity-selected filamentous bacteriophage as a probe for acoustic wave biodetectors of Salmonella typhimurium

• Published in: Biosensors & bioelectronics 2006-02, Vol.21 (8), p.1434-1442
• Main Authors: Olsen, Eric V., Sorokulova, Iryna B., Petrenko, Valery A., Chen, I-Hsuan, Barbaree, James M., Vodyanoy, Vitaly J.
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteriophages - isolation & purification; Bacteriophages - physiology; Biological and medical sciences; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Colony Count, Microbial - instrumentation; Colony Count, Microbial - methods; Electrochemistry - instrumentation; Electrochemistry - methods; Equipment Design; Equipment Failure Analysis; Filamentous bacteriophage; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Molecular Probe Techniques - instrumentation; Phage display; Piezoelectric; Quartz crystal microbalance; Reproducibility of Results; Salmonella typhimurium; Salmonella typhimurium - isolation & purification; Salmonella typhimurium - virology; Sensitivity and Specificity; Various methods and equipments
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2005.06.004

Proof-in-concept biosensors were prepared for the rapid detection of Salmonella typhimurium in solution, based on affinity-selected filamentous phage prepared as probes physically adsorbed to piezoelectric transducers. Quantitative deposition studies indicated that ≈3 × 10 10 phage particles/cm 2 could be irreversibly adsorbed for 1 h at room temperature to prepare working biosensors. The quality of phage deposition was monitored by fluorescent microscopy. Specific-bacterial binding resulted in resonance frequency changes of prepared sensors, which were evaluated using linear regression analysis. Sensors possessed a rapid response time of