Overexpression of the novel human gene, nuclear apoptosis-inducing factor 1, induces apoptosis

Apoptosis is a genetically determined cell suicidal program that plays critical roles in many physiological and pathological processes. In this study, we report the cloning and characterization of a novel human gene, nuclear apoptosis-inducing factor 1 ( NAIF1), overexpression of which induces apopt...

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Bibliographic Details
Published in:The international journal of biochemistry & cell biology 2006, Vol.38 (4), p.671-683
Main Authors: Lv, Bingfeng, Shi, Taiping, Wang, Xinyu, Song, Quansheng, Zhang, Yingmei, Shen, Yan, Ma, Dalong, Lou, Yaxin
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus - physiology
Animals
Apoptosis - genetics
Apoptosis Regulatory Proteins - biosynthesis
Apoptosis Regulatory Proteins - genetics
C9orf90
Cell Nucleus - genetics
Cell Nucleus - metabolism
Chromosomes, Human, Pair 9 - genetics
Chromosomes, Human, Pair 9 - metabolism
Gene Expression
HeLa Cells
Homeodomain-like region
Humans
Nuclear apoptosis-inducing factor 1
Nuclear Localization Signals - genetics
Nuclear Localization Signals - metabolism
Nuclear protein
Nuclear Proteins - biosynthesis
Nuclear Proteins - genetics
Programmed cell death
Transfection
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Summary:Apoptosis is a genetically determined cell suicidal program that plays critical roles in many physiological and pathological processes. In this study, we report the cloning and characterization of a novel human gene, nuclear apoptosis-inducing factor 1 ( NAIF1), overexpression of which induces apoptosis in cells. Human NAIF1 is located on chromosome 9q34.11 and encodes 327 amino acids with a homeodomain-like region and two nuclear localization signals at its N-terminal region. NAIF1 is conserved across diverse species, including human, mouse, crab-eating macaque, dog, chicken and frog, and shares no obvious homology to any known genes or proteins. Northern blot analysis revealed wide expression of NAIF1 mRNA throughout human tissues. NAIF1 was predominantly localized in the nucleus. Overexpression of NAIF1 inhibited cell growth and induced apoptosis. Furthermore, NAIF1 transfection caused both decreases in mitochondrial membrane potential and caspase-3 activation. In summary, NAIF1 is a nuclear protein that induces apoptosis when overexpressed.
ISSN:1357-2725
1878-5875
DOI:10.1016/j.biocel.2005.11.007