Differences in the API 20E biochemical patterns of clinical and environmental Vibrio parahaemolyticus isolates

Abstract Genetic differences in clinical and environmental strains of Vibrio parahaemolyticus have been widely used as criteria in identifying pathogenic isolates. However, few studies have been carried out to assess the differences in biochemical characteristics of V. parahaemolyticus isolates from...

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Bibliographic Details
Published in:FEMS microbiology letters 2006-02, Vol.255 (1), p.75-81
Main Authors: Martinez-Urtaza, Jaime, Lozano-Leon, Antonio, Viña-Feas, Alejandro, de Novoa, Jacobo, Garcia-Martin, Oscar
Format: Article
Language:English
Subjects:
Animals
API 20E
Arabinose
Bacterial Typing Techniques
Bacteriological Techniques
Biochemical characteristics
biochemical characterization
Biochemical tests
Biochemistry
Clinical isolates
Environmental Microbiology
food safety
Gelatin
Humans
Marine environment
Microbiology
Reagent Kits, Diagnostic
Seafood
Sodium chloride
tdh
Vibrio parahaemolyticus
Vibrio parahaemolyticus - classification
Vibrio parahaemolyticus - isolation & purification
Water Microbiology
Waterborne diseases
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Summary:Abstract Genetic differences in clinical and environmental strains of Vibrio parahaemolyticus have been widely used as criteria in identifying pathogenic isolates. However, few studies have been carried out to assess the differences in biochemical characteristics of V. parahaemolyticus isolates from human and environmental sources. We compared the biochemical profiles obtained by the characterization of V. parahaemolyticus isolates from human infections and the marine environment using the API 20E system. Environmental and clinical isolates showed significant differences in the gelatin and arabinose tests. Additionally, clinical isolates were correctly identified according to the API 20E profile using 0.85% NaCl diluent, but they presented nonspecific profiles with 2% NaCl diluent. In contrast, use of 2% NaCl diluent facilitated correct identification of the environmental isolates. Clinical isolates showed significant differences in up to five biochemical tests with respect to the API 20E database. The API 20E system is widely used in routine identification of bacteria in clinical laboratories, and this discrepancy in an important number of biochemical tests may lead to misidentification of V. parahaemolyticus infection.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2005.00052.x