INTERACTION OF EIMERIA TENELLA WITH INTESTINAL MUCIN IN VITRO

The mucus gel layer overlying the gastrointestinal epithelium plays an important role in host–pathogen interactions. The initial interaction between the coccidian parasite Eimeria tenella and host cells of the intestinal epithelium must occur across this mucus interface. In this study, we examined t...

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Bibliographic Details
Published in:The Journal of parasitology 2007-06, Vol.93 (3), p.634-638
Main Authors: Tierney, J. B, Matthews, E, Carrington, S. D, Mulcahy, G
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carbohydrates
Cattle
Cecum
Cecum - immunology
Cecum - parasitology
Cell Adhesion - immunology
Cell Line
Centrifugation
Centrifugation, Density Gradient - veterinary
Chickens
Dose-Response Relationship, Immunologic
Duodenum
Duodenum - immunology
Duodenum - parasitology
Eimeria tenella
Eimeria tenella - immunology
Eimeria tenella - pathogenicity
Epithelial cells
Epithelium
Fundamental and applied biological sciences. Psychology
Gel chromatography
General aspects
General aspects and techniques. Study of several systematic groups. Models
Glycoproteins
Host-pathogen interactions
Intestine
Intestines
Invertebrates
Mucin
Mucins
Mucins - immunology
Mucins - isolation & purification
Mucus
Parasite hosts
Parasites
Parasitology
PATHOLOGY
Poultry
Small intestine
Ungulates
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Summary:The mucus gel layer overlying the gastrointestinal epithelium plays an important role in host–pathogen interactions. The initial interaction between the coccidian parasite Eimeria tenella and host cells of the intestinal epithelium must occur across this mucus interface. In this study, we examined the relationship between E. tenella and avian mucin, in particular the effect of purified intestinal regional mucin on parasite adherence and invasion in vitro. Secreted mucin from the chicken duodenum and cecum was purified by density gradient centrifugation and gel chromatography. Parasite invasion studies were performed in the Madin-Darby bovine kidney cell model. Eimeria tenella adherence to chicken duodenal mucin was detected, whereas adherence to cecal or bovine mucin was not shown. Parasite invasion into epithelial cells was not influenced by bovine mucin, whereas chicken mucin purified from the duodenum and cecum significantly inhibited invasion. Inhibition of E. tenella invasion into cells by mucin from the duodenum was marginally greater than that of the cecum, but this was not significant. This study demonstrated E. tenella interaction with native chicken intestinal mucin, which in turn inhibited parasite invasion into epithelial cells in vitro.
ISSN:0022-3395
1937-2345
DOI:10.1645/GE-1066R.1