Evidence of human immunodeficiency virus type 1 infection of nestin-positive neural progenitors in archival pediatric brain tissue

Human immunodeficiency virus type 1 (HIV-1) central nervous system (CNS) infection in children is associated with impaired brain growth and neurodevelopmental delays. Neural progenitors are critical for neurogenesis. Human multipotential neural progenitors grown in culture are permissive for HIV-1 i...

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Bibliographic Details
Published in:Journal of neurovirology 2007-01, Vol.13 (3), p.274-283
Main Authors: Schwartz, Lynnae, Civitello, Lucy, Dunn-Pirio, Anastasie, Ryschkewitsch, Samantha, Berry, Elizabeth, Cavert, Winston, Kinzel, Nikilyn, Lawrence, Diane M P, Hazra, Rohan, Major, Eugene O
Format: Article
Language:English
Subjects:
AIDS Dementia Complex - pathology
Biological and medical sciences
Biomarkers - metabolism
Child, Preschool
DNA, Viral - metabolism
Female
HIV-1 - genetics
HIV-1 - isolation & purification
Human viral diseases
Humans
In Situ Hybridization
Infant
Infectious diseases
Intermediate Filament Proteins - metabolism
Male
Medical sciences
Microdissection
Nerve Tissue Proteins - metabolism
Nestin
Neurons - pathology
Stem Cells - metabolism
Stem Cells - pathology
Stem Cells - virology
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral diseases of the nervous system
Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye
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Summary:Human immunodeficiency virus type 1 (HIV-1) central nervous system (CNS) infection in children is associated with impaired brain growth and neurodevelopmental delays. Neural progenitors are critical for neurogenesis. Human multipotential neural progenitors grown in culture are permissive for HIV-1 infection, but it is not known if infection of these cells occurs in vivo. Brain tissue from pre-highly active antiretroviral therapy (HAART) era pediatric acquired immunodeficiency syndrome (AIDS) patients was examined for evidence of HIV-1 infection of nestin-positive neural progenitors by in situ hybridization; or after laser microdissection harvest, DNA extraction, and polymerase chain reaction (PCR). HIV-1 or viral DNA was identified in nestin-positive cells in four of seven HIV-1-infected children, suggesting in vivo infection of neural progenitors.
ISSN:1355-0284
1538-2443
DOI:10.1080/13550280701344975