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Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications
Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi–contained gelatin fibers were characterized usin...
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Published in: | Biomaterials 2006-05, Vol.27 (13), p.2705-2715 |
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description | Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi–contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to ∼5%
w/w), the average fiber size was reduced from 803±121
nm to 61±13
nm (
p
<
0.0
1
) and the tensile modulus increased from 499±207
MPa to 1384±105
MPa (
p
<
0.0
5
). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering. |
doi_str_mv | 10.1016/j.biomaterials.2005.11.037 |
format | article |
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w/w), the average fiber size was reduced from 803±121
nm to 61±13
nm (
p
<
0.0
1
) and the tensile modulus increased from 499±207
MPa to 1384±105
MPa (
p
<
0.0
5
). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2005.11.037</identifier><identifier>PMID: 16352335</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Aniline Compounds - analysis ; Aniline Compounds - chemistry ; Animals ; Biocompatible Materials - analysis ; Biocompatible Materials - chemistry ; Cell Culture Techniques - methods ; Cell Line ; Electrochemistry - methods ; Electrospinning ; Gelatin ; Gelatin - analysis ; Gelatin - chemistry ; H9c2 cardiac myoblasts ; Myoblasts - cytology ; Myoblasts - physiology ; Nanotubes - analysis ; Nanotubes - chemistry ; Nanotubes - ultrastructure ; Particle Size ; Polyaniline (PANi) ; Rats ; Rotation ; Tensile Strength ; Textiles ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2006-05, Vol.27 (13), p.2705-2715</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-2f9c7d3b8f9525642d15b6018acb90a1d47191f9ef94f9f657e1ee29b5118cfe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16352335$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Mengyan</creatorcontrib><creatorcontrib>Guo, Yi</creatorcontrib><creatorcontrib>Wei, Yen</creatorcontrib><creatorcontrib>MacDiarmid, Alan G.</creatorcontrib><creatorcontrib>Lelkes, Peter I.</creatorcontrib><title>Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi–contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to ∼5%
w/w), the average fiber size was reduced from 803±121
nm to 61±13
nm (
p
<
0.0
1
) and the tensile modulus increased from 499±207
MPa to 1384±105
MPa (
p
<
0.0
5
). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering.</description><subject>Aniline Compounds - analysis</subject><subject>Aniline Compounds - chemistry</subject><subject>Animals</subject><subject>Biocompatible Materials - analysis</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line</subject><subject>Electrochemistry - methods</subject><subject>Electrospinning</subject><subject>Gelatin</subject><subject>Gelatin - analysis</subject><subject>Gelatin - chemistry</subject><subject>H9c2 cardiac myoblasts</subject><subject>Myoblasts - cytology</subject><subject>Myoblasts - physiology</subject><subject>Nanotubes - analysis</subject><subject>Nanotubes - chemistry</subject><subject>Nanotubes - ultrastructure</subject><subject>Particle Size</subject><subject>Polyaniline (PANi)</subject><subject>Rats</subject><subject>Rotation</subject><subject>Tensile Strength</subject><subject>Textiles</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqNkcFq3DAQhkVpaTZpX6GYHHqzo5EsyeotJGlaCOTS3gpClkeLFq_kSt5C3j4Ku9De0pNG8P0zw3yEXALtgIK82nVjSHu7Yg52Lh2jVHQAHeXqDdnAoIZWaCrekg2FnrVaAjsj56XsaP3Tnr0nZyC5YJyLDfl1N6NbcypLiDHEbbOk-cnGMIeIrUtxtbWYmi3Odg2xiTYmH0bMpfEpN2so5YANxm2l6jo1b5dlDq7CKZYP5J2vG-LH03tBfn69-3HzrX14vP9-c_3QOiH52jKvnZr4OHgtmJA9m0CMksJg3aiphalXoMFr9Lr32kuhEBCZHgXA4DzyC_L52HfJ6fcBy2r2oTicZxsxHYpRVEE_CP0qyDRwpSR_FQTd94NktIJfjqCrNywZvVly2Nv8ZICaF1tmZ_61ZV5sGQBTbdXwp9OUw7jH6W_0pKcCt0cA6_X-BMymuIDR4RRy1WamFP5nzjPrhK87</recordid><startdate>20060501</startdate><enddate>20060501</enddate><creator>Li, Mengyan</creator><creator>Guo, Yi</creator><creator>Wei, Yen</creator><creator>MacDiarmid, Alan G.</creator><creator>Lelkes, Peter I.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7TB</scope><scope>7U5</scope><scope>F28</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20060501</creationdate><title>Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications</title><author>Li, Mengyan ; Guo, Yi ; Wei, Yen ; MacDiarmid, Alan G. ; Lelkes, Peter I.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-2f9c7d3b8f9525642d15b6018acb90a1d47191f9ef94f9f657e1ee29b5118cfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Aniline Compounds - analysis</topic><topic>Aniline Compounds - chemistry</topic><topic>Animals</topic><topic>Biocompatible Materials - analysis</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Line</topic><topic>Electrochemistry - methods</topic><topic>Electrospinning</topic><topic>Gelatin</topic><topic>Gelatin - analysis</topic><topic>Gelatin - chemistry</topic><topic>H9c2 cardiac myoblasts</topic><topic>Myoblasts - cytology</topic><topic>Myoblasts - physiology</topic><topic>Nanotubes - analysis</topic><topic>Nanotubes - chemistry</topic><topic>Nanotubes - ultrastructure</topic><topic>Particle Size</topic><topic>Polyaniline (PANi)</topic><topic>Rats</topic><topic>Rotation</topic><topic>Tensile Strength</topic><topic>Textiles</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Mengyan</creatorcontrib><creatorcontrib>Guo, Yi</creatorcontrib><creatorcontrib>Wei, Yen</creatorcontrib><creatorcontrib>MacDiarmid, Alan G.</creatorcontrib><creatorcontrib>Lelkes, Peter I.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Mengyan</au><au>Guo, Yi</au><au>Wei, Yen</au><au>MacDiarmid, Alan G.</au><au>Lelkes, Peter I.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2006-05-01</date><risdate>2006</risdate><volume>27</volume><issue>13</issue><spage>2705</spage><epage>2715</epage><pages>2705-2715</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Polyaniline (PANi), a conductive polymer, was blended with a natural protein, gelatin, and co-electrospun into nanofibers to investigate the potential application of such a blend as conductive scaffold for tissue engineering purposes. Electrospun PANi–contained gelatin fibers were characterized using scanning electron microscopy (SEM), electrical conductivity measurement, mechanical tensile testing, and differential scanning calorimetry (DSC). SEM analysis of the blend fibers containing less than 3% PANi in total weight, revealed uniform fibers with no evidence for phase segregation, as also confirmed by DSC. Our data indicate that with increasing the amount of PANi (from 0 to ∼5%
w/w), the average fiber size was reduced from 803±121
nm to 61±13
nm (
p
<
0.0
1
) and the tensile modulus increased from 499±207
MPa to 1384±105
MPa (
p
<
0.0
5
). The results of the DSC study further strengthen our notion that the doping of gelatin with a few % PANi leads to an alteration of the physicochemical properties of gelatin. To test the usefulness of PANi-gelatin blends as a fibrous matrix for supporting cell growth, H9c2 rat cardiac myoblast cells were cultured on fiber-coated glass cover slips. Cell cultures were evaluated in terms of cell proliferation and morphology. Our results indicate that all PANi-gelatin blend fibers supported H9c2 cell attachment and proliferation to a similar degree as the control tissue culture-treated plastic (TCP) and smooth glass substrates. Depending on the concentrations of PANi, the cells initially displayed different morphologies on the fibrous substrates, but after 1week all cultures reached confluence of similar densities and morphology. Taken together these results suggest that PANi-gelatin blend nanofibers might provide a novel conductive material well suited as biocompatible scaffolds for tissue engineering.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>16352335</pmid><doi>10.1016/j.biomaterials.2005.11.037</doi><tpages>11</tpages></addata></record> |
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subjects | Aniline Compounds - analysis Aniline Compounds - chemistry Animals Biocompatible Materials - analysis Biocompatible Materials - chemistry Cell Culture Techniques - methods Cell Line Electrochemistry - methods Electrospinning Gelatin Gelatin - analysis Gelatin - chemistry H9c2 cardiac myoblasts Myoblasts - cytology Myoblasts - physiology Nanotubes - analysis Nanotubes - chemistry Nanotubes - ultrastructure Particle Size Polyaniline (PANi) Rats Rotation Tensile Strength Textiles Tissue engineering Tissue Engineering - methods |
title | Electrospinning polyaniline-contained gelatin nanofibers for tissue engineering applications |
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