Smart Stability-Indicating Spectrophotometric Methods for Determination of Binary Mixtures Without Prior Separation

Ratio subtraction and isosbestic point methods are 2 innovating spectrophotometric methods used to determine vincamine in the presence of its acid degradation product and a mixture of cinnarizine (CN) and nicergoline (NIC). Linear correlations were obtained in the concentration range from 8-40 micro...

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Bibliographic Details
Published in:Journal of AOAC International 2008-03, Vol.91 (2), p.299-310
Main Authors: EL-BARDICY, Mohammad G, LOTFY, Hayam M, EL-SAYED, Mohammad A, EL-TARRAS, Mohammad F
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cinnarizine - analysis
Drug Stability
Drugs
Food industries
Fundamental and applied biological sciences. Psychology
Methods
Nicergoline - analysis
Product/Service Evaluations
Spectrophotometry
Spectrophotometry - methods
Vincamine - analysis
Vincamine - chemistry
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Summary:Ratio subtraction and isosbestic point methods are 2 innovating spectrophotometric methods used to determine vincamine in the presence of its acid degradation product and a mixture of cinnarizine (CN) and nicergoline (NIC). Linear correlations were obtained in the concentration range from 8-40 microg/mL for vincamine (I), 6-22 microg/mL for CN (II), and 6-36 microg/mL for NIC (III), with mean accuracies 99.72 +/- 0.917% for I, 99.91 +/- 0.703% for II, and 99.58 +/- 0.847 and 99.83 +/- 1.039% for III. The ratio subtraction method was utilized for the analysis of laboratory-prepared mixtures containing different ratios of vincamine and its degradation product, and it was valid in the presence of up to 80% degradation product. CN and NIC in synthetic mixtures were analyzed by the 2 proposed methods with the total content of the mixture determined at their respective isosbestic points of 270.2 and 235.8 nm, and the content of CN was determined by the ratio subtraction method. The proposed method was validated and found to be suitable as a stability-indicating assay method for vincamine in pharmaceutical formulations. The standard addition technique was applied to validate the results and to ensure the specificity of the proposed methods.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/91.2.299