Mutual regulation between serine and nitric oxide metabolism in human glioblastoma cells

• Published in: Neuroscience letters 2006-02, Vol.394 (3), p.163-167
• Main Authors: Shoji, Kazuo, Mariotto, Sofia, Ciampa, Anna Rosa, Suzuki, Hisanori
• Format: Article
• Language: English
• Subjects: Astrocyte; Biological and medical sciences; Blotting, Western; Brain Neoplasms - metabolism; Cell Line, Tumor; Cytosol - drug effects; Cytosol - metabolism; d-Amino acid oxidase (DAAO); d-Serine; Dose-Response Relationship, Drug; Fluorescent Dyes; Fundamental and applied biological sciences. Psychology; Glioblastoma - metabolism; Humans; Nitric Oxide - metabolism; Nitric oxide synthase (NOS); Nitric Oxide Synthase Type I - metabolism; Nitric Oxide Synthase Type III - metabolism; Receptor Cross-Talk - drug effects; Serine - metabolism; Serine racemase (SR); Vertebrates: nervous system and sense organs
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2005.10.064

d-Serine indirectly caused dose- and time-dependent inhibition of neuronal nitric oxide synthase (nNOS) without affecting endothelial nitric oxide synthase (eNOS) in human glioblastoma cell line U87. Activity of d-amino acid oxidase (DAAO), catalyzing the oxidative deamination of d-amino acid, was enhanced by NO in a dose-dependent manner. Recently, we have reported that serine racemase (SR) is inhibited by NO and activated by d-serine through nitrosylation and denitrosylation, respectively [K. Shoji, S. Mariotto, A.R. Ciampa, H. Suzuki, Regulation of serine racemase activity by d-serine and nitric oxide in human glioblastoma cells, Neurosci. Lett., in press]. Thus, the metabolism of both d-serine and NO in U87 cells is functionally correlated in a complex manner. Suppression of NO production by d-serine in U87 cells contrasts its known action in enhancing nNOS in neurons.