YHV-protease dsRNA inhibits YHV replication in Penaeus monodon and prevents mortality

Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Inject...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-03, Vol.341 (2), p.351-356
Main Authors: Yodmuang, Supansa, Tirasophon, Witoon, Roshorm, Yaowaluck, Chinnirunvong, Wanlop, Panyim, Sakol
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
Dose-Response Relationship, Drug
Double-stranded RNA
Hemolymph - metabolism
Penaeidae - genetics
Penaeidae - virology
Penaeus monodon
Plasmids - metabolism
Polymerase Chain Reaction
Recombinant Proteins - chemistry
Reverse Transcriptase Polymerase Chain Reaction
RNA Interference
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
Roniviridae - genetics
Roniviridae - metabolism
Time Factors
Virus Replication
Yellow head virus
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Summary:Yellow head virus infects cultured shrimps and causes severe mortality resulting in a great economic loss. Haemolymph injection of dsRNA(pro) corresponding to the protease motif of YHV genome resulted in a complete inhibition of YHV replication. The effect of dsRNA lasted for at least 5 days. Injecting sequence-unrelated dsRNA(gfp) or dsRNA(TSV-pol) also resulted in an inhibition of YHV replication but at a comparatively much less extent. Shrimp mortality was monitored for 10 days when more than 90% shrimps receiving no dsRNA died within 8 dpi. However, those receiving dsRNA(pro) showed no mortality. A partial mortality was observed among the shrimps receiving dsRNA(gfp) or dsRNA(TSV-pol). Thus, Penaeus monodon possesses the sequence-specific protection to YHV infection, most likely through the RNAi pathway, in addition to sequence-independent protection. It gives a new notion that dsRNA induction of antiviral immunity in shrimp goes through two pathways, sequence-independent and sequence-dependent.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2005.12.186