Proteome analysis identifies novel protein candidates involved in regeneration of the cerebellum of teleost fish

In contrast to mammals, adult teleost fish exhibit an enormous potential to regenerate neuronal tissue after injuries to the CNS. By combining a well‐defined cerebellar lesion paradigm with differential proteome analysis at a post‐lesion survival time of 3 days, we screened for protein candidates in...

Full description

Saved in:
Bibliographic Details
Published in:Proteomics (Weinheim) 2006-01, Vol.6 (2), p.677-696
Main Authors: Zupanc, Marianne M., Wellbrock, Ursula M., Zupanc, Günther K. H.
Format: Article
Language:English
Subjects:
Adult neurogenesis
Analytical, structural and metabolic biochemistry
Animals
Apteronotus leptorhynchus
Axonal regeneration
Biological and medical sciences
Blotting, Western
Cerebellum - injuries
Cerebellum - metabolism
Cerebellum - pathology
Electrophoresis, Gel, Two-Dimensional
Fish Proteins - metabolism
Fishes - metabolism
Fundamental and applied biological sciences. Psychology
Miscellaneous
Nerve Regeneration - physiology
Neuronal regeneration
Proteins
Proteome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Teleostei
Wound healing
Wound Healing - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In contrast to mammals, adult teleost fish exhibit an enormous potential to regenerate neuronal tissue after injuries to the CNS. By combining a well‐defined cerebellar lesion paradigm with differential proteome analysis at a post‐lesion survival time of 3 days, we screened for protein candidates involved in repair of the fish brain. Out of nearly 900 protein spots detected on 2‐D gels, spot intensity was significantly increased at least twofold in 30 spots and decreased to at least half the intensity of control tissue in 23 spots. The proteins associated with 24 of the spots were identified by PMF and MS/MS fragmentation. The cellular localization and the spatiotemporal patterns of two of these proteins, beta‐actin and beta‐tubulin, were further characterized through immunohistochemistry. Comparison of the observed changes in protein abundance with the previously characterized events underlying regeneration of the cerebellum suggests that the proteins identified are especially involved in cellular proliferation and survival, as well as axonal sprouting.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200500167