Column Reversed-Phase High-Performance Liquid Chromatographic Method for Simultaneous Determination of Rabeprazole Sodium and Domperidone in Combined Tablet Dosage Form

A new, simple column reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of rabeprazole sodium (RAB) and domperidone (DOM) in a combined tablet dosage form has been developed and validated. Determination was performed using a Jasco HPLC system with a H...

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Bibliographic Details
Published in:Journal of AOAC International 2008-03, Vol.91 (2), p.344-348
Main Authors: Sabnis, Shweta Sadanand, Dnvandev, Dhavale Nilesh, Jadhav, Vijay Yeshawantrao, Gandhi, Santosh Vilashchand
Format: Article
Language:English
Subjects:
2-Pyridinylmethylsulfinylbenzimidazoles - analysis
Antiemetics
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Domperidone - analysis
Drug Combinations
Drugs
Food industries
Fundamental and applied biological sciences. Psychology
High performance liquid chromatography
Identification and classification
Measurement
Methods
Product/Service Evaluations
Rabeprazole
Tablets
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Summary:A new, simple column reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of rabeprazole sodium (RAB) and domperidone (DOM) in a combined tablet dosage form has been developed and validated. Determination was performed using a Jasco HPLC system with a HiQ SiL octadecylsilane (C18) column (250 x 4.6 mm id), acetonitrile-0.1 M ammonium acetate (50 + 50, v/v) mobile phase, and paracetamol as an internal standard. The detection was performed using a UV detector set at 280 nm. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 1.0-10.0 and 0.5-5.0 microg/mL for RAB and DOM, respectively. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/91.2.344