Phytase from antarctic yeast strain Cryptococcus laurentii AL27

Cryptococcus laurentii strain AL 27 demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH 2 PO 4 providing 5 mg/L phosphorus, and cultivat...

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Bibliographic Details
Published in:Folia microbiologica 2008, Vol.53 (1), p.29-34
Main Authors: Pavlova, K., Gargova, S., Hristozova, T., Tankova, Z.
Format: Article
Language:English
Subjects:
6-Phytase - chemistry
6-Phytase - genetics
6-Phytase - metabolism
Antarctic Regions
Applied Microbiology
Biomedical and Life Sciences
Cryptococcus - enzymology
Cryptococcus - genetics
Cryptococcus - isolation & purification
Cryptococcus - metabolism
Environmental Engineering/Biotechnology
Enzyme Stability
Fungal Proteins - chemistry
Fungal Proteins - genetics
Fungal Proteins - metabolism
Immunology
Kinetics
Life Sciences
Microbiology
Soil Microbiology
Temperature
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Summary:Cryptococcus laurentii strain AL 27 demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH 2 PO 4 providing 5 mg/L phosphorus, and cultivation temperature of 24 °C, which relates it to psychrotrophic microorganisms. The enzyme kinetic characteristics according to sodium phytate were K m = 0.98 mmol/L, v lim = 33.3 μmol g −1 min −1 . The enzyme had maximum activity at 40 °C and acted within a wide pH range: from 2.0 to 5.5, which is of positive significance for its direct inclusion into the feed of monogastric animals.
ISSN:0015-5632
1874-9356
DOI:10.1007/s12223-008-0004-3