Estimation of prognoses for cervical intraepithelial neoplasia 2 by p16INK4a immunoexpression and high-risk HPV in situ hybridization signal types

The present study used immunohistochemical staining and in situ hybridization (ISH) to examine whether progression of cervical intraepithelial neoplasia, grade 2 (CIN 2) can be predicted by p16INK4a immunoexpression and high-risk human papilloma virus (HPV) ISH signal types. We studied 52 cases hist...

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Bibliographic Details
Published in:American journal of clinical pathology 2007-08, Vol.128 (2), p.208-217
Main Authors: OMORI, Makiko, HASHI, Akihiko, NAKAZAWA, Kumiko, YUMINAMOCHI, Tsutomu, YAMANE, Tetsu, HIRATA, Shuji, KATOH, Ryohei, HOSHI, Kazuhiko
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Cervical Intraepithelial Neoplasia - chemistry
Cervical Intraepithelial Neoplasia - mortality
Cervical Intraepithelial Neoplasia - virology
Cyclin-Dependent Kinase Inhibitor p16 - analysis
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Immunohistochemistry
In Situ Hybridization
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Middle Aged
Papillomaviridae - isolation & purification
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Prognosis
Tumors
Uterine Cervical Neoplasms - chemistry
Uterine Cervical Neoplasms - mortality
Uterine Cervical Neoplasms - virology
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Summary:The present study used immunohistochemical staining and in situ hybridization (ISH) to examine whether progression of cervical intraepithelial neoplasia, grade 2 (CIN 2) can be predicted by p16INK4a immunoexpression and high-risk human papilloma virus (HPV) ISH signal types. We studied 52 cases histologically diagnosed with CIN 2: dysplasia regressed in 28 cases; 13 cases progressed to CIN 3; and CIN 2 persisted in 11 cases. Expression of p16INK4a and high-risk HPV signal both related to grade of CIN. Stronger p16INK4a immunoexpression and a higher frequency of expression of a punctate nuclear signal were observed in CIN 2 lesions before progression compared with those before regression. CIN 2 cases in which moderate to strong immunoexpression of p16INK4a and a punctate signal were observed simultaneously progressed to CIN 3 in 10 (91%) of 11 cases. CIN 2 cases with moderate to strong immunoexpression of p16INK4a and a high-risk HPV punctate signal should be treated because of the great risk of progression.
ISSN:0002-9173
1943-7722