Expression of oxidative stress-related molecules in circulating leukocytes and urine in patients with chronic viral hepatitis

: Aims: Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress‐related molecules remains to be clarified. Methods: 4‐hydroxy‐2‐nonenal (4‐HNE), 4‐hydroxy‐2‐hexenal (4‐HHE), catalase, superoxide dismutase‐1 (SOD‐1), glutathione peroxidase‐1, thioredox...

Full description

Saved in:
Bibliographic Details
Published in:Liver international 2006-03, Vol.26 (2), p.157-165
Main Authors: Saeki, Toshiya, Ichiba, Miho, Tanabe, Naotada, Ueki, Masaru, Okamoto, Kinya, Matsunaga, Yoshiko, Hosho, Keiko, Kanbe, Takamasa, Tsuchiya, Hiroyuki, Kurimasa, Akihiro, Yamada, Sadako, Hirooka, Yasuaki, Hisatome, Ichiro, Kishimoto, Yukihiro, Suou, Takeaki, Murawaki, Yoshikazu, Kawasaki, Hironaka, Yodoi, Junji, Shiota, Goshi
Format: Article
Language:English
Subjects:
Aged
Alanine Transaminase - blood
Aldehydes - metabolism
antioxidant protein
Aspartate Aminotransferases - blood
Blotting, Western
chronic viral hepatitis
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - urine
Enzyme-Linked Immunosorbent Assay
Enzymes
Female
Hepatitis, Viral, Human - blood
Hepatitis, Viral, Human - pathology
Hepatitis, Viral, Human - urine
Humans
leukocyte
Leukocytes - metabolism
Leukocytes - pathology
Lipid Peroxidation
Liver - metabolism
Liver - pathology
Male
Middle Aged
Oxidative Stress
Thioredoxins - metabolism
Up-Regulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: Aims: Oxidative stress plays a role in pathogenesis of chronic viral hepatitis. Expression of oxidative stress‐related molecules remains to be clarified. Methods: 4‐hydroxy‐2‐nonenal (4‐HNE), 4‐hydroxy‐2‐hexenal (4‐HHE), catalase, superoxide dismutase‐1 (SOD‐1), glutathione peroxidase‐1, thioredoxin (TRX) in leukocytes and urinary 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) were examined in 164 persons, including 130 chronic viral hepatitis patients and 34 normal individuals, by Western blot analysis and enzyme‐linked immunosorbent assay, respectively. Hepatic expression of these proteins was immunohistochemically examined in 12 patients with chronic viral hepatitis, compared with three persons without liver damage. Results: The 4‐HNE/β‐actin ratios in chronic viral hepatitis were significantly higher than those in normal individuals (P
ISSN:1478-3223
1478-3231
DOI:10.1111/j.1478-3231.2005.01213.x