Strategies for comprehensive analysis of amino acid biomarkers of oxidative stress

Despite the wide interest in using modified amino acids as putative biomarkers of oxidative stress, many issues remain as to their overall reliability for early detection and diagnosis of diseases. In contrast to conventional single biomarker studies, comprehensive analysis of biomarkers offers an u...

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Bibliographic Details
Published in:Amino acids 2007-07, Vol.33 (1), p.3-18
Main Authors: Ptolemy, A. S, Lee, R, Britz-McKibbin, P
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - analysis
analytical methods
Assessments
Biomarkers
Biomarkers - analysis
Comprehensive analysis
Diagnosis
Humans
Mathematical analysis
Metabolism
Modified amino acids
Multiple biomarkers
Oxidative Stress
Primary Prevention
Reactive Nitrogen Species - analysis
Reactive Oxygen Species - analysis
Strategy
Stresses
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Summary:Despite the wide interest in using modified amino acids as putative biomarkers of oxidative stress, many issues remain as to their overall reliability for early detection and diagnosis of diseases. In contrast to conventional single biomarker studies, comprehensive analysis of biomarkers offers an unbiased strategy for global assessment of modified amino acid metabolism due to reactive oxygen and nitrogen species. This review examines recent analytical techniques amenable for analysis of modified amino acids in biological samples reported during 2003-2007. Particular attention is devoted to the need for validated methods applicable to high-throughput analysis of multiple amino acid biomarkers, as well as consideration of sample pretreatment protocols on artifact formation for improved clinical relevance.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-007-0542-z