Detection of Tuber melanosporum DNA in soil

Abstract Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PC...

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Bibliographic Details
Published in:FEMS microbiology letters 2006-01, Vol.254 (2), p.251-257
Main Authors: Suz, Laura M., Martín, María P., Colinas, Carlos
Format: Article
Language:English
Subjects:
Ascomycota - genetics
Ascomycota - growth & development
Ascomycota - isolation & purification
Biological and medical sciences
Deoxyribonucleic acid
DNA
DNA Primers
DNA, Fungal - analysis
DNA, Fungal - isolation & purification
Fundamental and applied biological sciences. Psychology
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Hyphae
ITS
Microbiology
Mycelia
Mycology
PCR‐RFLP
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
Quercus
Sensitivity analysis
Soil - analysis
Soil Microbiology
soil mycelium
Soil testing
Soils
Trees
truffle orchard
Tuber melanosporum
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Summary:Abstract Our objectives were (i) to develop a molecular method to detect mycelia of Tuber melanosporum (black truffle) in soil and (ii) to test for mycelial distribution around two truffle-bearing Quercus ilex trees in a truffle orchard. Isolation of total DNA from soil was performed, followed by PCR amplification with T. melanosporum-specific primers and restriction analysis. To address the detection sensitivity level, soil samples were inoculated with known amounts of gleba of T. melanosporum. The detection limit was ≥11.4 μg of hyphae g−1 of soil. Mycelium was detected primarily within the area defined by the truffle burn and within the top 35 cm of the soil in all directions from the trees.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2005.00029.x