Liquid chromatography-tandem mass spectrometric method for determination of the anti-inflammatory compound vicenin-2 in the leaves of L. ericoides Mart

• Published in: Biomedical chromatography 2007-09, Vol.21 (9), p.925-930
• Main Authors: Moraes, Solange Leite de, Tomaz, José Carlos, Lopes, Norberto Peporine
• Format: Article
• Language: English
• Subjects: Anti-Inflammatory Agents - analysis; Apigenin - analysis; Asteraceae - chemistry; Chromatography, Liquid - methods; ESI-MS/MS; Glucosides - analysis; LC-MS/MS; natural product; Plant Extracts - chemistry; Plant Leaves - chemistry; quantification; Sensitivity and Specificity; Spectrometry, Mass, Electrospray Ionization - methods; Tandem Mass Spectrometry - methods; vicenin-2
• ISSN: 0269-3879; 1099-0801
• DOI: 10.1002/bmc.828

This paper reports a rapid and sensitive method for determination of the anti‐inflammatory compound vicenin‐2 in L. ericoides Mart. using liquid chromatography–tandem mass spectrometry. Separation of the compound of interest was performed on a VP‐ODS18 (150 × 2 mm, Shimadzu, Japan) column and a pre‐column packed with GPV‐ODS C18 (5 × 2 mm, Sigma‐Aldrich, USA) with acetonitrile–water (15:85) mobile phase containing 2% acetic acid using isocratic flow at 0.5 mL/min for 2 min. Multiple‐reaction monitoring of vicenin‐2 was performed using electrospray positive ionization. The linear calibration curves were generated using a concentration range of 5–2500 ng/mL with correlation coefficients >0.99. The values of limit of detection and limit of quantitation were found to be 1 and 5 ng/mL, respectively. The method developed based on LC‐ESIMS/MS is advantageous because it permits the rapid and selective detection of vicenin‐2. Furthermore, the method can be easily applied to the routine analysis of vicenin‐2 in plant extracts using a minimal amount of sample. Copyright © 2007 John Wiley & Sons, Ltd.