Cloning and molecular characterization of a gene encoding a Cryptosporidium parvum putative 20S proteasome beta1-type subunit

A DNA sequence composed of 1281 nucleotides (nt) consisting of a single open reading frame (ORF) encoding a putative 20S proteasome beta1-type subunit was isolated from clones derived from genomic libraries constructed from the KSU-1 isolate of Cryptosporidium parvum. Southern blot analysis suggeste...

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Bibliographic Details
Published in:DNA sequence 2000, Vol.11 (3-4), p.309-314
Main Authors: Chung, P A, Johnson, J, Khramtsov, N V, Upton, S J
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Cryptosporidium parvum - genetics
Cysteine Endopeptidases - chemistry
Cysteine Endopeptidases - genetics
Molecular Sequence Data
Multienzyme Complexes - chemistry
Multienzyme Complexes - genetics
Open Reading Frames
Proteasome Endopeptidase Complex
Protein Subunits
Restriction Mapping
Saccharomyces cerevisiae - chemistry
Saccharomyces cerevisiae - genetics
Sequence Alignment
Sequence Homology, Amino Acid
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Summary:A DNA sequence composed of 1281 nucleotides (nt) consisting of a single open reading frame (ORF) encoding a putative 20S proteasome beta1-type subunit was isolated from clones derived from genomic libraries constructed from the KSU-1 isolate of Cryptosporidium parvum. Southern blot analysis suggested that the sequenced DNA exists in the C. parvum genome as a single copy; transcription was verified through reverse transcription-polymerase chain reaction (RT-PCR) performed on total RNA isolated from C. parvum sporozoites. The predicted protein consists of 210 amino acids (aa), contains characteristic amino acids common to all proteasomal subunits, and shares stronger similarity to the beta1-type subunit of yeast than to other types of beta-subunits.
ISSN:1042-5179