Rcs phosphorelay modulates the expression of plant cell wall degrading enzymes and virulence in Pectobacterium carotovorum ssp. carotovorum

Production of plant cell wall degrading enzymes, the major virulence factors of soft-rot Pectobacterium species, is controlled by many regulatory factors. Pectobacterium carotovorum ssp. carotovorum SCC3193 encodes an Rcs phosphorelay system that involves two sensor kinases, RcsCPcc and RcsDPcc, and...

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Bibliographic Details
Published in:FEMS microbiology letters 2007-08, Vol.273 (2), p.229-238
Main Authors: Andresen, Liis, Kõiv, Viia, Alamäe, Tiina, Mäe, Andres
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
Biological and medical sciences
Cell walls
Deactivation
Degradation
Enzymes
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Inactivation
Kinases
Microbiology
Models, Biological
mutants
Mutation
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Pectobacterium
Pectobacterium carotovorum
Pectobacterium carotovorum - enzymology
Pectobacterium carotovorum - genetics
Pectobacterium carotovorum - pathogenicity
Phosphorylation
phytopathogenic bacteria
plant pathogenic bacteria
Rcs phosphorelay
Rot
Signal Transduction
two-component system
Virulence
Virulence factors
Virulence Factors - genetics
Virulence Factors - metabolism
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Summary:Production of plant cell wall degrading enzymes, the major virulence factors of soft-rot Pectobacterium species, is controlled by many regulatory factors. Pectobacterium carotovorum ssp. carotovorum SCC3193 encodes an Rcs phosphorelay system that involves two sensor kinases, RcsCPcc and RcsDPcc, and a response regulator RcsBPcc as key components of this system, and an additional small lipoprotein RcsFPcc. This study indicates that inactivation of rcsCPcc, rcsDPcc and rcsBPcc enhances production of virulence factors with the highest effect detected for rcsBPcc. Interestingly, mutation of rcsFPcc has no effect on virulence factors synthesis. These results suggest that in SCC3193 a parallel phosphorylation mechanism may activate the RcsBPcc response regulator, which acts as a repressor suppressing the plant cell wall degrading enzyme production. Enhanced production of virulence factors in Rcs mutants is more pronounced when bacteria are growing in the absence of plant signal components.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2007.00794.x