Usefulness of the determination of IgG and IgM antibodies by ELISA and immunocapture in a clinical series of human brucellosis

The diagnostic difficulties of brucellosis makes the evaluation of new diagnostic tests necessary. Evaluation of different commercial tests in the serological diagnosis of brucellosis by ELISA and immunocapture antibodies in a clinical series of patients with brucellosis of the Health Network of the...

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Bibliographic Details
Published in:Revista chilena de infectología 2008-04, Vol.25 (2), p.116-121
Main Authors: Aranís J, Carolina, Oporto C, Jorge, Espinoza, Mónica, Riedel K, Ingrid, Pérez C, Carlos, García C, Patricia
Format: Article
Language:Spanish
Subjects:
Acute Disease
Adult
Aged
Agglutination Tests - methods
Brucella - immunology
Brucellosis - diagnosis
Brucellosis - immunology
Case-Control Studies
Chronic Disease
Enzyme-Linked Immunosorbent Assay - methods
Female
Humans
Immunoglobulin G - blood
Immunoglobulin M - blood
Male
Middle Aged
Reproducibility of Results
Retrospective Studies
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Summary:The diagnostic difficulties of brucellosis makes the evaluation of new diagnostic tests necessary. Evaluation of different commercial tests in the serological diagnosis of brucellosis by ELISA and immunocapture antibodies in a clinical series of patients with brucellosis of the Health Network of the Catholic University of Chile. All the serums received in the Laboratory of Microbiology for suspicion of brucellosis during five years were studied. Two groups were obtained, one that fulfilled diagnostic criteria for brucellosis [clinical evidence, and/or positive blood culture and/or seroagglutination test (SAT) in titers > 1/160] and the control group. Each serum sample was analyzed using irnmunocapture-agglutination test (Brucellacapt), ELISA IgM and IgG. Of 10 patients with brucellosis, the serologic results were: 8/10 positives for ELISA IgG, 7/10 for Brucellacapt and SAT, and 5/10 for ELISA IgM. ELISA IgG alone was superior than SAT. The combination ELISA IgG/ Brucellacapt reaches the best detection performance (9/10) and can be an alternative to SAT.
ISSN:0716-1018