Protein-Phosphorylation-Responsive Polymeric Nanoparticles for Imaging Protein Kinase Activities in Single Living Cells

Take your PIC: Cell‐permeable and biocompatible polyion‐induced complex (PIC) nanoparticles were developed consisting of a negatively charged polymer along with a positively charged polymer conjugated to a near‐infrared fluorophore and a protein‐kinase‐specific peptide. Owing to profound changes in...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2007-01, Vol.46 (30), p.5779-5782
Main Authors: Kim, Jong-Ho, Lee, Seulki, Park, Kyeongsoon, Nam, Hae Yun, Jang, Soon Young, Youn, Inchan, Kim, Kwangmeyung, Jeon, Hyesung, Park, Rang-Woon, Kim, In-San, Choi, Kuiwon, Kwon, Ick Chan
Format: Article
Language:English
Subjects:
Animals
cell imaging
Cell Survival
CHO Cells
Cricetinae
Cricetulus
fluorescent probes
Microscopy, Fluorescence
Molecular Structure
nanoparticles
Nanoparticles - chemistry
Phosphorylation
Polymers - chemistry
protein kinases
Protein Kinases - metabolism
sensors
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Summary:Take your PIC: Cell‐permeable and biocompatible polyion‐induced complex (PIC) nanoparticles were developed consisting of a negatively charged polymer along with a positively charged polymer conjugated to a near‐infrared fluorophore and a protein‐kinase‐specific peptide. Owing to profound changes in fluorescence, the PIC nanoparticle is an attractive probe for protein kinase A (PKA) in single living cells. ATP=adenosine triphosphate, ADP=adenosine diphosphate.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200700767