Differential Expression of Alternatively Spliced mRNAs of Arabidopsis SR Protein Homologs, atSR30 and atSR45a, in Response to Environmental Stress

Serine/arginine-rich (SR) proteins are associated with either the regulation or the execution of both constitutive splicing and the selection of alternative splice sites in animals and plants. We demonstrated the molecular characterization of a homolog of SR protein, atSR45a, in Arabidopsis plants....

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Bibliographic Details
Published in:Plant and cell physiology 2007-07, Vol.48 (7), p.1036-1049
Main Authors: Tanabe, Noriaki, Yoshimura, Kazuya, Kimura, Ayako, Yabuta, Yukinori, Shigeoka, Shigeru
Format: Article
Language:English
Subjects:
Alternative splicing
Alternative Splicing - genetics
Arabidopsis
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Carrier Proteins - genetics
Carrier Proteins - metabolism
Environment
Gene Expression Regulation, Plant
High-light irradiation
Mutation
RNA, Messenger - genetics
RNA, Messenger - metabolism
RNA, Plant - genetics
RNA, Plant - metabolism
RNA-Binding Proteins
SR protein
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Summary:Serine/arginine-rich (SR) proteins are associated with either the regulation or the execution of both constitutive splicing and the selection of alternative splice sites in animals and plants. We demonstrated the molecular characterization of a homolog of SR protein, atSR45a, in Arabidopsis plants. Six types of mRNA variants (atSR45a-1a-e and atSR45a-2) were generated by the alternative selection of transcriptional initiation sites and the alternative splicing of introns in atSR45a pre-mRNA. The atSR45a-1a and -2 proteins, presumed mature forms, were located in the nucleus and interacted with U1-70K, suggesting that these proteins function as a splicing factor in ARABIDOPSIS: The levels of the transcripts atSR45a and atSR30, SF2/ASF-like SR proteins, were increased by various types of stress, such as high-light irradiation and salinity. Furthermore, the splicing patterns of atSR45a and atSR30 pre-mRNA themselves were altered under these stressful conditions. In particular, the expression of atSR45a-1a, atSR45a-2, atSR30 mRNA1 and atSR30 mRNA3 was greatly increased by high-light irradiation. These results indicate that the regulation of transcription and alternative splicing of atSR45a and atSR30 is responsive to various stressful conditions.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcm069