Effects of Low Power Laser Irradiation on Intracellular Calcium and Histamine Release in RBL-2H3 Mast Cells

Although laser irradiation has been reported to promote skin wound healing, the mechanism is still unclear. As mast cells are found to accumulate at the site of skin wounds we hypothesized that mast cells might be involved in the biological effects of laser irradiation. In this work the mast cells,...

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Bibliographic Details
Published in:Photochemistry and photobiology 2007-07, Vol.83 (4), p.979-984
Main Authors: Yang, Wen-Zhong, Chen, Ji-Yao, Yu, Ji-Tong, Zhou, Lu-Wei
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Cell Line, Tumor
Cells
Fluorescence
Histamine
Histamine Release - radiation effects
Lasers
Mast Cells - metabolism
Mast Cells - radiation effects
Proteins
Rats
Spectrometry, Fluorescence
TRPV Cation Channels - metabolism
Wound healing
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Summary:Although laser irradiation has been reported to promote skin wound healing, the mechanism is still unclear. As mast cells are found to accumulate at the site of skin wounds we hypothesized that mast cells might be involved in the biological effects of laser irradiation. In this work the mast cells, RBL‐2H3, were used in vitro to investigate the effects of laser irradiation on cellular responses. After laser irradiation, the amount of intracellular calcium ([Ca2+]i) was increased, followed by histamine release, as measured by confocal fluorescence microscopy with Fluo‐3/AM staining and a fluorescence spectrometer with o‐phthalaldehyde staining, respectively. The histamine release was mediated by the increment of [Ca2+]i from the influx of the extracellular buffer solution through the cation channel protein, transient receptor potential vanilloid 4 (TRPV4). The TRPV4 inhibitor, Ruthenium Red (RR) can effectively block such histamine release, indicating that TRPV4 was the key factor responding to laser irradiation. These induced responses of mast cells may provide an explanation for the biological effects of laser irradiation on promoting wound healing, as histamine is known to have multi‐functions on accelerating wound healing.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.2007.00116.x