Dematin and Adducin Provide a Novel Link between the Spectrin Cytoskeleton and Human Erythrocyte Membrane by Directly Interacting with Glucose Transporter-1

Dematin and adducin are actin-binding proteins located at the spectrin-actin junctions, also called the junctional complex, in the erythrocyte membrane. Here we propose a new model whereby dematin and adducin link the junctional complex to human erythrocyte plasma membrane. Using a combination of su...

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Bibliographic Details
Published in:The Journal of biological chemistry 2008-05, Vol.283 (21), p.14600-14609
Main Authors: Khan, Anwar A., Hanada, Toshihiko, Mohseni, Morvarid, Jeong, Jong-Jin, Zeng, Lixiao, Gaetani, Massimiliano, Li, Donghai, Reed, Brent C., Speicher, David W., Chishti, Athar H.
Format: Article
Language:English
Subjects:
Animals
Blood Proteins - genetics
Blood Proteins - metabolism
Calmodulin-Binding Proteins - metabolism
Cell Line
Cytoskeleton - metabolism
Erythrocyte Membrane - metabolism
Glucose Transporter Type 1 - genetics
Glucose Transporter Type 1 - metabolism
Humans
Mice
Protein Binding
Proteomics
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Summary:Dematin and adducin are actin-binding proteins located at the spectrin-actin junctions, also called the junctional complex, in the erythrocyte membrane. Here we propose a new model whereby dematin and adducin link the junctional complex to human erythrocyte plasma membrane. Using a combination of surface labeling, immunoprecipitation, and vesicle proteomics approaches, we have identified glucose transporter-1 as the receptor for dematin and adducin in the human erythrocyte membrane. This finding is the first description of a transmembrane protein that binds to dematin and adducin, thus providing a rationale for the attachment of the junctional complex to the lipid bilayer. Because homologues of dematin, adducin, and glucose transporter-1 exist in many non-erythroid cells, we propose that a conserved mechanism may exist that couples sugar and other related transporters to the actin cytoskeleton.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M707818200