Analysis and Occurrence of 14 Sulfonamide Antibacterials and Chloramphenicol in Honey by Solid-Phase Extraction Followed by LC/MS/MS Analysis

A method was developed for the analysis of 14 sulfonamide antibiotics and chloramphenicol in honey. These antibiotics have been banned for use in food-producing animals; yet, their residues were found in many samples, illustrating the need for a multiresidue analysis for these antibiotics in honey....

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2008-05, Vol.56 (10), p.3509-3516
Main Authors: Sheridan, Robert, Policastro, Brian, Thomas, Stefan, Rice, Daniel
Format: Article
Language:English
Subjects:
Analytical Methods
Anti-Bacterial Agents - analysis
antibacterial properties
antibiotics
Biological and medical sciences
chloramphenicol
Chloramphenicol - analysis
chloramphenocol
Chromatography, Liquid
Confectionery products and chocolate industries, honey
detection
drug residues
food composition
food contamination
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
honey
Honey - analysis
LC/MS/MS
liquid chromatography
mass spectrometry
solid phase extraction
Sulfonamide
sulfonamides
Sulfonamides - analysis
Tandem Mass Spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method was developed for the analysis of 14 sulfonamide antibiotics and chloramphenicol in honey. These antibiotics have been banned for use in food-producing animals; yet, their residues were found in many samples, illustrating the need for a multiresidue analysis for these antibiotics in honey. The method described here uses an acid hydrolysis step to liberate the sugar-bound sulfonamides followed by a solid-phase extraction to remove potential interferences. Analysis was by liquid chromatography−electrospray ionization−tandem mass spectrometry in negative mode for all 15 analytes. This MRM method generated two structurally significant transitions per compound, and it was designed to conform to U.S. Food and Drug Administration MS confirmation guidelines. It also provides 4-EU identification points. One hundred sixteen samples from 25 countries were analyzed, and 38% were found to contain at least one target antimicrobial. Five different target compounds were found in honey from 13 different countries.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf800293m