Diagnostic value of micro-CT in comparison with histology in the qualitative assessment of historical human skull bone pathologies

• Published in: American journal of physical anthropology 2007-08, Vol.133 (4), p.1099-1111
• Main Authors: Rühli, F.J., Kuhn, G., Evison, R., Müller, R., Schultz, M.
• Format: Article
• Language: English
• Subjects: Anthropological methodology; Biological anthropology; Bone Diseases - diagnostic imaging; Bone Diseases - history; Bone Diseases - pathology; Bone industry; Craniocerebral Trauma - diagnostic imaging; Craniocerebral Trauma - history; Craniocerebral Trauma - pathology; Diseases; Evolutionary anthropology; Generalities; History, 20th Century; Human remains; Humans; hyperostosis frontalis interna; Hyperostosis Frontalis Interna - diagnostic imaging; Hyperostosis Frontalis Interna - history; Hyperostosis Frontalis Interna - pathology; hyperparathyroidism; Hyperparathyroidism - diagnostic imaging; Hyperparathyroidism - history; Hyperparathyroidism - pathology; Imaging, Three-Dimensional - methods; Methodology and general studies; Microscopy, Polarization; Organisation and history of research; osteomyelitis; Osteomyelitis - diagnostic imaging; Osteomyelitis - history; Osteomyelitis - pathology; Paleopathology; Physical anthropology; Prehistory and protohistory; Primary Myelofibrosis - diagnostic imaging; Primary Myelofibrosis - history; Primary Myelofibrosis - pathology; Qualitative analysis; Skull - diagnostic imaging; Skull - pathology; syphilis; Syphilis - diagnostic imaging; Syphilis - history; Syphilis - pathology; Tomography, X-Ray Computed - methods; Typology, technology and attribute analysis
• ISSN: 0002-9483; 1096-8644
• DOI: 10.1002/ajpa.20611

Cases of pathologically changed bone might constitute a diagnostic pitfall and frequently need histological methods to be etiologically properly evaluated. With micro‐computed tomography (μCT), a new epoch of 2D and 3D imaging has been launched. We evaluated the diagnostic investigation of this analytical method versus well established histological investigations of historical human bone. Pathological changes due to various etiologies (infectious, traumatic, endocrinological, neoplasia) observed in autopsy‐based macerated human skulls (Galler Collection, Natural History Museum Basel, Switzerland) were investigated by μCT and compared with histological thin ground sections using polarized light. Micro‐CT images visualize the architecture of the bone with high spatial resolution without preparation or destruction of the sample in the area to be sectioned. Changes in the bone surfaces as well as alterations of the diploë can be assessed. However, morphological patterns caused by reactive response, such as typical arrangements of collagen fibers, can only be visualized by the microscopic investigation of thin ground sections using polarized light. A great advantage of μCT is the high number of slices obtained so that spatial differences within the areas of the specimen become visible. Micro‐CT is a valuable tool for the diagnosis of vestiges of skull bone diseases. Its advantages over histology are the fast, automated image acquisition and the fact that the specimen is not completely destroyed. Only excision of the area to be scanned is necessary, if the specimen is too large to be scanned as a whole. Further, the 3D visualization of the micro‐architecture allows an easy orientation within the sample, for example, for the choice of the location of the histological slices. However, the need to differentiate woven from lamellar bone still makes histology an indispensable method. Am J Phys Anthropol, 2007. © 2007 Wiley‐Liss, Inc.