Phytoecdysteroids Increase Protein Synthesis in Skeletal Muscle Cells

Phytoecdysteroids, which are structurally similar or identical to insect molting hormones, produce a range of effects in mammals, including increasing growth and physical performance. To study the mechanism of action of phytoecdysteroids in mammalian tissue, an in vitro cellular assay of protein syn...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2008-05, Vol.56 (10), p.3532-3537
Main Authors: Gorelick-Feldman, Jonathan, MacLean, David, Ilic, Nebojsa, Poulev, Alexander, Lila, Mary Ann, Cheng, Diana, Raskin, Ilya
Format: Article
Language:English
Subjects:
ajuga
Ajuga - chemistry
animal models
animal performance
Animals
Bioactive Constituents
bioassays
Biological and medical sciences
C2C12
cell culture
Cell Line
dosage
Ecdysteroid
Ecdysteroids - analysis
Ecdysteroids - pharmacology
Ecdysterone - pharmacology
enzyme inhibitors
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
human cell lines
Humans
in vitro studies
kinases
Male
mammals
Mice
Muscle Cells - drug effects
Muscle Cells - metabolism
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
myotube
phosphoinositide kinase-3
phytoecdysteroids
plant extracts
Plants - chemistry
Protein Biosynthesis - drug effects
protein synthesis
Rats
Rats, Sprague-Dawley
skeletal muscle
spinach
Spinacia oleracea - chemistry
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Summary:Phytoecdysteroids, which are structurally similar or identical to insect molting hormones, produce a range of effects in mammals, including increasing growth and physical performance. To study the mechanism of action of phytoecdysteroids in mammalian tissue, an in vitro cellular assay of protein synthesis was developed. In C2C12 murine myotubes and human primary myotubes, phytoecdysteroids increased protein synthesis by up to 20%. In vivo, ecdysteroids increased rat grip strength. Ecdysteroid-containing plant extracts produced similar results. The effect was inhibited by a phosphoinositide kinase-3 inhibitor, which suggests a PI3K-mediated mechanism.
ISSN:0021-8561
1520-5118
DOI:10.1021/jf073059z