Characterization of the rrn operons in the channel catfish pathogen Edwardsiella ictaluri

To advance diagnostics and phylogenetics of Edwardsiella ictaluri by sequencing and characterizing its rrn operons. The Edw. ictaluri rrn operons were identified from a 5-7 kbp insert lambda library and from Edw. ictaluri fosmid clones. We present the complete sequences and analysis of all eight Edw...

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Bibliographic Details
Published in:Journal of applied microbiology 2008-06, Vol.104 (6), p.1790-1796
Main Authors: Williams, M.L, Waldbieser, G.C, Dyer, D.W, Gillaspy, A.F, Lawrence, M.L
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
channel catfish
DNA Primers - genetics
Edwardsiella ictaluri
Edwardsiella ictaluri - genetics
Edwardsiella tarda
Edwardsiella tarda - genetics
Electrophoresis, Gel, Pulsed-Field - methods
Enterobacteriaceae
Enterobacteriaceae Infections - diagnosis
Fish Diseases - diagnosis
Fish Diseases - microbiology
Fisheries
Freshwater
Fundamental and applied biological sciences. Psychology
Genomic Library
Ictaluridae - microbiology
Ictalurus punctatus
Industrial Microbiology
Microbiology
Molecular Sequence Data
PCR
Polymerase Chain Reaction - methods
pulsed field gel electrophoresis
ribosomal DNA operon
rRNA Operon - genetics
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Summary:To advance diagnostics and phylogenetics of Edwardsiella ictaluri by sequencing and characterizing its rrn operons. The Edw. ictaluri rrn operons were identified from a 5-7 kbp insert lambda library and from Edw. ictaluri fosmid clones. We present the complete sequences and analysis of all eight Edw. ictaluri rrn operons and unique regions located upstream and downstream. Two rrn operons were located in tandem with 169 bp separating them, which is apparently a conserved feature between Edw. ictaluri and Edwardsiella tarda. I-CeuI enzyme digestion of Edw. ictaluri genomic DNA and analysis by pulsed field gel electrophoresis indicated that rrn operon number and chromosomal locations are conserved within the species Edw. ictaluri. The rrn operons of Edw. ictaluri have similar structure and flanking regions compared with other members of the family Enterobacteriaceae; however, the presence of eight copies of the rrn operon makes Edw. ictaluri unique within the family. This research clarifies previous phylogenetic analyses of Edw. ictaluri and provides support for the Edw. ictaluri genome sequencing project. In addition, we identified a unique feature of two rrn operons that shows potential for the development of a diagnostic PCR method.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2007.03704.x