Polyunsaturated fatty acids production with a solid-state column reactor

• Published in: Bioresource technology 2008-09, Vol.99 (14), p.6181-6189
• Main Authors: Jang, Hung-Der, Yang, Shang-Shyng
• Format: Article
• Language: English
• Subjects: aeration; alpha-linolenic acid; arachidonic acid; Biological and medical sciences; Biotechnology; carbon; Column reactor; eicosapentaenoic acid; Fatty Acids, Unsaturated - biosynthesis; Food industries; Fundamental and applied biological sciences. Psychology; linoleic acid; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Mortierella; Mortierella - metabolism; Mortierella alpina; nitrogen; Nitrogen - administration & dosage; Oryza - metabolism; Oryza sativa; Polyunsaturated fatty acid; Rice bran; Solid-state fermentation; temperature; Use and upgrading of agricultural and food by-products. Biotechnology; water content
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2007.12.024

To investigate the potential production of polyunsaturated fatty acids (PUFAs), a solid-state column reactor of rice bran with Mortierella alpina was used. The optimal conditions for PUFAs production were rice bran supplementation with 3.75% (w w −1) nitrogen source at initial moisture content 57%, initial pH 6–7, aeration, and incubation at 20 °C for 5 days and then at 12 °C for 7 days. Each gram of substrate carbon yielded 127 mg of total PUFAs, 12 mg of eicosapentaenoic acid (EPA), 6 mg of arachidonic acid (AA), 5 mg of α-linolenic acid (ALA), and 117 mg of linoleic acid (LA) after 12 days incubation. Aeration enhanced the productions of AA, EPA, and total PUFAs. Supplementation of the nitrogen source on the fourth day and then a shift to lower temperature on the fifth day increased EPA production.