Instability Studies of Porcine Somatotropin in Aqueous Solutions and the Possible Reagents for Its Stabilization

The instability of porcine somatotropin (pST) in various solutions and possible stabilization of the hormone by sugars and mild detergents were studied. Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel perme...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 2000-11, Vol.48 (11), p.5685-5691
Main Authors: Fan, Kesuo, Sevoian, Martin, Gonzales, Denis
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Drug Stability
Excipients - chemistry
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
Glucose
Growth Hormone - chemistry
Hydrogen-Ion Concentration
Recombinant Proteins - chemistry
Sodium Dodecyl Sulfate
Solutions
Sucrose
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The instability of porcine somatotropin (pST) in various solutions and possible stabilization of the hormone by sugars and mild detergents were studied. Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel permeation chromatography (GPC) or ultraviolet spectroscopy (UV). The pST is a very unstable hormone in an aqueous environment. It was found in this project that the peptide hormone underwent aggregation or decomposition quickly in acidic and alkaline solutions but slowly in neutral pH solutions. High losses of pST monomers were seen in concentrated solutions of the hormone. On the other hand, pST monomers were stabilized to a certain degree in glucose solutions and at a low concentration of urea. These results should facilitate the development of efficient controlled-release systems which are essential for commercializing porcine somatotropin. Keywords: Porcine somatotropin; aggregation; stabilization; controlled release
ISSN:0021-8561
1520-5118
DOI:10.1021/jf990801p