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Instability Studies of Porcine Somatotropin in Aqueous Solutions and the Possible Reagents for Its Stabilization

The instability of porcine somatotropin (pST) in various solutions and possible stabilization of the hormone by sugars and mild detergents were studied. Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel perme...

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Published in:	Journal of agricultural and food chemistry 2000-11, Vol.48 (11), p.5685-5691
Main Authors:	Fan, Kesuo, Sevoian, Martin, Gonzales, Denis
Format:	Article
Language:	English
Subjects:	Animals Biological and medical sciences Drug Stability Excipients - chemistry Feed and pet food industries Food industries Fundamental and applied biological sciences. Psychology Glucose Growth Hormone - chemistry Hydrogen-Ion Concentration Recombinant Proteins - chemistry Sodium Dodecyl Sulfate Solutions Sucrose Swine
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container_title	Journal of agricultural and food chemistry
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creator	Fan, Kesuo Sevoian, Martin Gonzales, Denis
description	The instability of porcine somatotropin (pST) in various solutions and possible stabilization of the hormone by sugars and mild detergents were studied. Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel permeation chromatography (GPC) or ultraviolet spectroscopy (UV). The pST is a very unstable hormone in an aqueous environment. It was found in this project that the peptide hormone underwent aggregation or decomposition quickly in acidic and alkaline solutions but slowly in neutral pH solutions. High losses of pST monomers were seen in concentrated solutions of the hormone. On the other hand, pST monomers were stabilized to a certain degree in glucose solutions and at a low concentration of urea. These results should facilitate the development of efficient controlled-release systems which are essential for commercializing porcine somatotropin. Keywords: Porcine somatotropin; aggregation; stabilization; controlled release
doi_str_mv	10.1021/jf990801p
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Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel permeation chromatography (GPC) or ultraviolet spectroscopy (UV). The pST is a very unstable hormone in an aqueous environment. It was found in this project that the peptide hormone underwent aggregation or decomposition quickly in acidic and alkaline solutions but slowly in neutral pH solutions. High losses of pST monomers were seen in concentrated solutions of the hormone. On the other hand, pST monomers were stabilized to a certain degree in glucose solutions and at a low concentration of urea. These results should facilitate the development of efficient controlled-release systems which are essential for commercializing porcine somatotropin. 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Agric. Food Chem</addtitle><description>The instability of porcine somatotropin (pST) in various solutions and possible stabilization of the hormone by sugars and mild detergents were studied. Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel permeation chromatography (GPC) or ultraviolet spectroscopy (UV). The pST is a very unstable hormone in an aqueous environment. It was found in this project that the peptide hormone underwent aggregation or decomposition quickly in acidic and alkaline solutions but slowly in neutral pH solutions. High losses of pST monomers were seen in concentrated solutions of the hormone. On the other hand, pST monomers were stabilized to a certain degree in glucose solutions and at a low concentration of urea. These results should facilitate the development of efficient controlled-release systems which are essential for commercializing porcine somatotropin. 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Psychology</subject><subject>Glucose</subject><subject>Growth Hormone - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Recombinant Proteins - chemistry</subject><subject>Sodium Dodecyl Sulfate</subject><subject>Solutions</subject><subject>Sucrose</subject><subject>Swine</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNptkE1r3DAQhkVpaLZpD_0DRVBayMGpJFuSfQyhHwspSeoUehNjWWq19VqOJEPTX18ZL9tLYWAYzTOjd16EXlFyQQmj73e2aUhN6PQEbShnpOCU1k_RhuRmUXNBT9HzGHeEkJpL8gydUkpqyctmg6btGBN0bnDpEbdp7p2J2Ft864N2o8Gt30PyKfjJjTjH5cNs_Bzz-zAn58eIYexx-mnyRIyuGwz-auCHGVPE1ge8zbldP_gDy8ALdGJhiOblIZ-hbx8_3F99Lq5vPm2vLq8LKKVMhWC9pGXVNRWzwCWV0BBitGBWlqJnwnLKWKUJ6KXubA20EqXpJBOsthrKM_Ru3TsFnzXHpPYuajMMMC4HKEmkqAShGTxfQR3yBcFYNQW3h_CoKFGLvepob2ZfH5bO3d70_8iDnxl4cwAgahhsgFG7eOTqimfdmSpWysVkfh-7EH4pIUvJ1f1tq9r67ju541_Uwr9dedBR7fwcxuzcf-T9BTAmndw</recordid><startdate>20001101</startdate><enddate>20001101</enddate><creator>Fan, Kesuo</creator><creator>Sevoian, Martin</creator><creator>Gonzales, Denis</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20001101</creationdate><title>Instability Studies of Porcine Somatotropin in Aqueous Solutions and the Possible Reagents for Its Stabilization</title><author>Fan, Kesuo ; Sevoian, Martin ; Gonzales, Denis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a377t-62d7134b942fa5717a900ec62f736d26f51224c0ac736dbf8a1463eb72628fca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Drug Stability</topic><topic>Excipients - chemistry</topic><topic>Feed and pet food industries</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose</topic><topic>Growth Hormone - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Recombinant Proteins - chemistry</topic><topic>Sodium Dodecyl Sulfate</topic><topic>Solutions</topic><topic>Sucrose</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fan, Kesuo</creatorcontrib><creatorcontrib>Sevoian, Martin</creatorcontrib><creatorcontrib>Gonzales, Denis</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fan, Kesuo</au><au>Sevoian, Martin</au><au>Gonzales, Denis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Instability Studies of Porcine Somatotropin in Aqueous Solutions and the Possible Reagents for Its Stabilization</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2000-11-01</date><risdate>2000</risdate><volume>48</volume><issue>11</issue><spage>5685</spage><epage>5691</epage><pages>5685-5691</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The instability of porcine somatotropin (pST) in various solutions and possible stabilization of the hormone by sugars and mild detergents were studied. Aggregation and decomposition of the hormone molecules in various pH solutions and under presence of sugar or detergent were monitored by gel permeation chromatography (GPC) or ultraviolet spectroscopy (UV). The pST is a very unstable hormone in an aqueous environment. It was found in this project that the peptide hormone underwent aggregation or decomposition quickly in acidic and alkaline solutions but slowly in neutral pH solutions. High losses of pST monomers were seen in concentrated solutions of the hormone. On the other hand, pST monomers were stabilized to a certain degree in glucose solutions and at a low concentration of urea. These results should facilitate the development of efficient controlled-release systems which are essential for commercializing porcine somatotropin. Keywords: Porcine somatotropin; aggregation; stabilization; controlled release</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>11087539</pmid><doi>10.1021/jf990801p</doi><tpages>7</tpages></addata></record>
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source	American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects	Animals Biological and medical sciences Drug Stability Excipients - chemistry Feed and pet food industries Food industries Fundamental and applied biological sciences. Psychology Glucose Growth Hormone - chemistry Hydrogen-Ion Concentration Recombinant Proteins - chemistry Sodium Dodecyl Sulfate Solutions Sucrose Swine
title	Instability Studies of Porcine Somatotropin in Aqueous Solutions and the Possible Reagents for Its Stabilization
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