Quantitation of canine regulatory T cell populations, serum interleukin-10 and allergen-specific IgE concentrations in healthy control dogs and canine atopic dermatitis patients receiving allergen-specific immunotherapy

• Published in: Veterinary immunology and immunopathology 2008-06, Vol.123 (3), p.337-344
• Main Authors: Keppel, K.E., Campbell, K.L., Zuckermann, F.A., Greeley, E.A., Schaeffer, D.J., Husmann, R.J.
• Format: Article
• Language: English
• Subjects: allergens; Animals; Atopic dermatitis; CD4-positive T-lymphocytes; chemical concentration; Cytokine; cytokines; Dermatitis, Atopic - immunology; Dermatitis, Atopic - therapy; Dermatitis, Atopic - veterinary; Desensitization, Immunologic - veterinary; dog diseases; Dog Diseases - blood; Dog Diseases - immunology; Dog Diseases - therapy; Dogs; Enzyme-Linked Immunosorbent Assay - veterinary; Female; Flow Cytometry - veterinary; immune response; immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunotherapy; interleukin-10; Interleukin-10 - blood; Interleukin-10 - immunology; Lymphocytes; Male; quantitative analysis; regulatory T cells; Skin Tests - veterinary; T-lymphocytes; T-Lymphocytes, Regulatory - immunology
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2008.02.008

Canine atopic dermatitis (AD) shares many clinical and immunological similarities with human AD. Regulatory T cells (Treg) are a distinct lineage of T lymphocytes with various immunosuppressive properties including the down-regulation of allergic inflammation associated with IgE production. Antigen-induced Treg typically regulate immune homeostasis via productions of cytokines such as interleukin-10. Given the immunological similarities with human AD, it is likely that Tregs and the cytokines they produce play an important role in diseases of dogs as well. A cross-reactive FoxP3 antibody was used to identify a subset of CD4 + T cells in the blood of both healthy dogs and dogs with atopic dermatitis undergoing immunotherapy over a year period. There was no significant difference in the Treg percentage over time in the healthy dogs. The immunotherapy group showed a significant increase in Treg percentage at 6, 9, and 12 months when compared to the healthy dogs. For the immunotherapy group, the mean Treg percentage at the beginning of the study was 4.94 ± 0.71 and 10.86 ± 2.73 at the completion. A commercially available ELISA kit was also used to quantitate the concentration of IL-10 in the serum of the same subsets of dogs. There was no significant difference in the IL-10 concentrations over time in the healthy dogs. The immunotherapy group showed a significant increase in serum IL-10 concentrations at 6, 9, and 12 months when compared to the control group. The mean serum IL-10 concentration at the initiation of immunotherapy was 20.40 ± 3.52 ng L −1 and 37.26 ± 15.26 ng L −1 at the completion of the study. The immunotherapy group also showed a significant decrease in serum IgE levels over the 1-year treatment period for specific allergens identified during ASIT. We conclude from these studies that similar to humans undergoing immunotherapy, increasing Treg populations likely play a significant role in the success of this particular type of therapy for atopic dermatitis and other allergic conditions.