Immune response to autologous transfusion in healthy volunteers: WB versus packed RBCs and FFP

BACKGROUND: Storage of blood as packed RBCs and FFP is standard practice in allogeneic transfusion. Separation into components has been proposed for autologous transfusion, as well, but beneficial effects have not yet been shown. STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were ran...

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Bibliographic Details
Published in:Transfusion (Philadelphia, Pa.) Pa.), 2001-04, Vol.41 (4), p.470-476
Main Authors: Frietsch, Thomas, Fessler, Heiko, Kirschfink, Michael, Nebe, Thomas, Waschke, Klaus F., Lorentz, Arnulf
Format: Article
Language:English
Subjects:
Adolescent
Adult
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Blood Transfusion, Autologous
Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis
Erythrocyte Transfusion
fMLP = N-formyl-Meth-Leu-Phe
FSC = forward scatter
Humans
Immunity
Male
Medical sciences
Middle Aged
Plasma
Plasma Exchange
PMA = phorbol-12-myristat-13-acetate
RCP group = volunteers receiving RBCs and FFP
SSC = side scatter
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
TRIM = transfusion-related immunomodulation
WB = whole blood
WB group = volunteers receiving WB
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Summary:BACKGROUND: Storage of blood as packed RBCs and FFP is standard practice in allogeneic transfusion. Separation into components has been proposed for autologous transfusion, as well, but beneficial effects have not yet been shown. STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were randomly assigned to receive 1 unit of either autologous RBCs and FFP (RCP group) or WB (WB group) after 49 or 35 days of storage, respectively. The immune response was analyzed by ELISA for IL‐6, C3a, terminal complement complex SC5b‐9, TNF‐α, and neopterin. Differential WBC counts and the phagocytosis of neutrophils and monocytes were measured by flow cytometry. RESULTS: Cell counts of monocytes (0.85 × 103 ng/mL) and neutrophils (6.9 × 103 ng/mL) increased 30 minutes after WB transfusion and then returned to close to the baseline values seen in the RCP group (0.47 and 2.9 × 103 ng/mL, respectively) throughout the monitored period (p
ISSN:0041-1132
1537-2995
DOI:10.1046/j.1537-2995.2001.41040470.x