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Immune response to autologous transfusion in healthy volunteers: WB versus packed RBCs and FFP
BACKGROUND: Storage of blood as packed RBCs and FFP is standard practice in allogeneic transfusion. Separation into components has been proposed for autologous transfusion, as well, but beneficial effects have not yet been shown. STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were ran...
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| Published in: | Transfusion (Philadelphia, Pa.) Pa.), 2001-04, Vol.41 (4), p.470-476 |
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| container_end_page | 476 |
| container_issue | 4 |
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| container_title | Transfusion (Philadelphia, Pa.) |
| container_volume | 41 |
| creator | Frietsch, Thomas Fessler, Heiko Kirschfink, Michael Nebe, Thomas Waschke, Klaus F. Lorentz, Arnulf |
| description | BACKGROUND: Storage of blood as packed RBCs and FFP is standard practice in allogeneic transfusion. Separation into components has been proposed for autologous transfusion, as well, but beneficial effects have not yet been shown.
STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were randomly assigned to receive 1 unit of either autologous RBCs and FFP (RCP group) or WB (WB group) after 49 or 35 days of storage, respectively. The immune response was analyzed by ELISA for IL‐6, C3a, terminal complement complex SC5b‐9, TNF‐α, and neopterin. Differential WBC counts and the phagocytosis of neutrophils and monocytes were measured by flow cytometry.
RESULTS: Cell counts of monocytes (0.85 × 103 ng/mL) and neutrophils (6.9 × 103 ng/mL) increased 30 minutes after WB transfusion and then returned to close to the baseline values seen in the RCP group (0.47 and 2.9 × 103 ng/mL, respectively) throughout the monitored period (p |
| doi_str_mv | 10.1046/j.1537-2995.2001.41040470.x |
| format | article |
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STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were randomly assigned to receive 1 unit of either autologous RBCs and FFP (RCP group) or WB (WB group) after 49 or 35 days of storage, respectively. The immune response was analyzed by ELISA for IL‐6, C3a, terminal complement complex SC5b‐9, TNF‐α, and neopterin. Differential WBC counts and the phagocytosis of neutrophils and monocytes were measured by flow cytometry.
RESULTS: Cell counts of monocytes (0.85 × 103 ng/mL) and neutrophils (6.9 × 103 ng/mL) increased 30 minutes after WB transfusion and then returned to close to the baseline values seen in the RCP group (0.47 and 2.9 × 103 ng/mL, respectively) throughout the monitored period (p<0.05). C3a (169 vs. 116 ng/mL) and IL‐6 (29 vs. 6 pg/mL) reached higher plasma concentrations in the WB group (n = 11) than in the RCP group (n = 10). Phagocytosis of opsonized Escherichia coli was increased in neutrophils and monocytes and lasted up to 7 days after the transfusion of whole blood.
CONCLUSION: Autologous WB induces a modest immunomodulation, but this effect is not observed upon transfusion of autologous blood components.</description><identifier>ISSN: 0041-1132</identifier><identifier>EISSN: 1537-2995</identifier><identifier>DOI: 10.1046/j.1537-2995.2001.41040470.x</identifier><identifier>PMID: 11316896</identifier><identifier>CODEN: TRANAT</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Inc</publisher><subject>Adolescent ; Adult ; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Biological and medical sciences ; Blood Transfusion, Autologous ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Erythrocyte Transfusion ; fMLP = N-formyl-Meth-Leu-Phe ; FSC = forward scatter ; Humans ; Immunity ; Male ; Medical sciences ; Middle Aged ; Plasma ; Plasma Exchange ; PMA = phorbol-12-myristat-13-acetate ; RCP group = volunteers receiving RBCs and FFP ; SSC = side scatter ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; TRIM = transfusion-related immunomodulation ; WB = whole blood ; WB group = volunteers receiving WB</subject><ispartof>Transfusion (Philadelphia, Pa.), 2001-04, Vol.41 (4), p.470-476</ispartof><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3590-c3e42925fb822e3e85506a654eb65080960897a5dcaa2796783c2e98d1e75e2a3</citedby><cites>FETCH-LOGICAL-c3590-c3e42925fb822e3e85506a654eb65080960897a5dcaa2796783c2e98d1e75e2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=959146$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11316896$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frietsch, Thomas</creatorcontrib><creatorcontrib>Fessler, Heiko</creatorcontrib><creatorcontrib>Kirschfink, Michael</creatorcontrib><creatorcontrib>Nebe, Thomas</creatorcontrib><creatorcontrib>Waschke, Klaus F.</creatorcontrib><creatorcontrib>Lorentz, Arnulf</creatorcontrib><title>Immune response to autologous transfusion in healthy volunteers: WB versus packed RBCs and FFP</title><title>Transfusion (Philadelphia, Pa.)</title><addtitle>Transfusion</addtitle><description>BACKGROUND: Storage of blood as packed RBCs and FFP is standard practice in allogeneic transfusion. Separation into components has been proposed for autologous transfusion, as well, but beneficial effects have not yet been shown.
STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were randomly assigned to receive 1 unit of either autologous RBCs and FFP (RCP group) or WB (WB group) after 49 or 35 days of storage, respectively. The immune response was analyzed by ELISA for IL‐6, C3a, terminal complement complex SC5b‐9, TNF‐α, and neopterin. Differential WBC counts and the phagocytosis of neutrophils and monocytes were measured by flow cytometry.
RESULTS: Cell counts of monocytes (0.85 × 103 ng/mL) and neutrophils (6.9 × 103 ng/mL) increased 30 minutes after WB transfusion and then returned to close to the baseline values seen in the RCP group (0.47 and 2.9 × 103 ng/mL, respectively) throughout the monitored period (p<0.05). C3a (169 vs. 116 ng/mL) and IL‐6 (29 vs. 6 pg/mL) reached higher plasma concentrations in the WB group (n = 11) than in the RCP group (n = 10). Phagocytosis of opsonized Escherichia coli was increased in neutrophils and monocytes and lasted up to 7 days after the transfusion of whole blood.
CONCLUSION: Autologous WB induces a modest immunomodulation, but this effect is not observed upon transfusion of autologous blood components.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Biological and medical sciences</subject><subject>Blood Transfusion, Autologous</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Erythrocyte Transfusion</subject><subject>fMLP = N-formyl-Meth-Leu-Phe</subject><subject>FSC = forward scatter</subject><subject>Humans</subject><subject>Immunity</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Plasma</subject><subject>Plasma Exchange</subject><subject>PMA = phorbol-12-myristat-13-acetate</subject><subject>RCP group = volunteers receiving RBCs and FFP</subject><subject>SSC = side scatter</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>TRIM = transfusion-related immunomodulation</subject><subject>WB = whole blood</subject><subject>WB group = volunteers receiving WB</subject><issn>0041-1132</issn><issn>1537-2995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqVkF1v0zAUhi3ExLqNv4AsTeIu4diJ7RiuWKFj0hhjGqrEBZabnLB0SdzFydb--zlqV6658cfxc17bDyGnDGIGqfywjJlIVMS1FjEHYHEaypAqiNevyGR_9ppMAFIWMZbwQ3Lk_RIAuAb2hhyGGpOZlhPy56JphhZph37lWo-0d9QOvavdXzd42ne29eXgK9fSqqV3aOv-bkMfXT20PWLnP9L5GX0MiwCvbH6PBb05m3pq24LOZtcn5KC0tce3u_mY_Jp9vZ1-iy5_nF9MP19GeSI0hBFTrrkoFxnnmGAmBEgrRYoLKSADLSHTyooit5YrLVWW5Bx1VjBUArlNjsn7be6qcw8D-t40lc-xrm2L4R9GgZJKpyyAn7Zg3jnvOyzNqqsa220MAzPqNUszKjSjQjPqNS96zTp0v9tdMywaLP717nwG4HQHWJ_bugz68srvOS00S0fqy5Z6qmrc_M8LzO3N7GUXYqJtTOV7XO9jbHdvpEqUMPOrc6O_X_1kcvrbzJNnQzylkQ</recordid><startdate>200104</startdate><enddate>200104</enddate><creator>Frietsch, Thomas</creator><creator>Fessler, Heiko</creator><creator>Kirschfink, Michael</creator><creator>Nebe, Thomas</creator><creator>Waschke, Klaus F.</creator><creator>Lorentz, Arnulf</creator><general>Blackwell Science Inc</general><general>Blackwell Publishing</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200104</creationdate><title>Immune response to autologous transfusion in healthy volunteers: WB versus packed RBCs and FFP</title><author>Frietsch, Thomas ; Fessler, Heiko ; Kirschfink, Michael ; Nebe, Thomas ; Waschke, Klaus F. ; Lorentz, Arnulf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3590-c3e42925fb822e3e85506a654eb65080960897a5dcaa2796783c2e98d1e75e2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Biological and medical sciences</topic><topic>Blood Transfusion, Autologous</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Erythrocyte Transfusion</topic><topic>fMLP = N-formyl-Meth-Leu-Phe</topic><topic>FSC = forward scatter</topic><topic>Humans</topic><topic>Immunity</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Plasma</topic><topic>Plasma Exchange</topic><topic>PMA = phorbol-12-myristat-13-acetate</topic><topic>RCP group = volunteers receiving RBCs and FFP</topic><topic>SSC = side scatter</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>TRIM = transfusion-related immunomodulation</topic><topic>WB = whole blood</topic><topic>WB group = volunteers receiving WB</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frietsch, Thomas</creatorcontrib><creatorcontrib>Fessler, Heiko</creatorcontrib><creatorcontrib>Kirschfink, Michael</creatorcontrib><creatorcontrib>Nebe, Thomas</creatorcontrib><creatorcontrib>Waschke, Klaus F.</creatorcontrib><creatorcontrib>Lorentz, Arnulf</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transfusion (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frietsch, Thomas</au><au>Fessler, Heiko</au><au>Kirschfink, Michael</au><au>Nebe, Thomas</au><au>Waschke, Klaus F.</au><au>Lorentz, Arnulf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune response to autologous transfusion in healthy volunteers: WB versus packed RBCs and FFP</atitle><jtitle>Transfusion (Philadelphia, Pa.)</jtitle><addtitle>Transfusion</addtitle><date>2001-04</date><risdate>2001</risdate><volume>41</volume><issue>4</issue><spage>470</spage><epage>476</epage><pages>470-476</pages><issn>0041-1132</issn><eissn>1537-2995</eissn><coden>TRANAT</coden><abstract>BACKGROUND: Storage of blood as packed RBCs and FFP is standard practice in allogeneic transfusion. Separation into components has been proposed for autologous transfusion, as well, but beneficial effects have not yet been shown.
STUDY DESIGN AND METHODS: Twenty‐four healthy male volunteers were randomly assigned to receive 1 unit of either autologous RBCs and FFP (RCP group) or WB (WB group) after 49 or 35 days of storage, respectively. The immune response was analyzed by ELISA for IL‐6, C3a, terminal complement complex SC5b‐9, TNF‐α, and neopterin. Differential WBC counts and the phagocytosis of neutrophils and monocytes were measured by flow cytometry.
RESULTS: Cell counts of monocytes (0.85 × 103 ng/mL) and neutrophils (6.9 × 103 ng/mL) increased 30 minutes after WB transfusion and then returned to close to the baseline values seen in the RCP group (0.47 and 2.9 × 103 ng/mL, respectively) throughout the monitored period (p<0.05). C3a (169 vs. 116 ng/mL) and IL‐6 (29 vs. 6 pg/mL) reached higher plasma concentrations in the WB group (n = 11) than in the RCP group (n = 10). Phagocytosis of opsonized Escherichia coli was increased in neutrophils and monocytes and lasted up to 7 days after the transfusion of whole blood.
CONCLUSION: Autologous WB induces a modest immunomodulation, but this effect is not observed upon transfusion of autologous blood components.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Inc</pub><pmid>11316896</pmid><doi>10.1046/j.1537-2995.2001.41040470.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Transfusion (Philadelphia, Pa.), 2001-04, Vol.41 (4), p.470-476 |
| issn | 0041-1132 1537-2995 |
| language | eng |
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| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Adolescent Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Biological and medical sciences Blood Transfusion, Autologous Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Erythrocyte Transfusion fMLP = N-formyl-Meth-Leu-Phe FSC = forward scatter Humans Immunity Male Medical sciences Middle Aged Plasma Plasma Exchange PMA = phorbol-12-myristat-13-acetate RCP group = volunteers receiving RBCs and FFP SSC = side scatter Transfusions. Complications. Transfusion reactions. Cell and gene therapy TRIM = transfusion-related immunomodulation WB = whole blood WB group = volunteers receiving WB |
| title | Immune response to autologous transfusion in healthy volunteers: WB versus packed RBCs and FFP |
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