Dimorphism, Thermal Tolerance, Virulence and Heat Shock Protein 70 Transcription in Different Isolates of Paracoccidioides brasiliensis

The mycelia-to-yeast (M-Y) transition, thermal tolerance and virulence profiles were evaluated for nine isolates of Paracoccidioides brasiliensis, including samples from two of the three recently discovered cryptic species, as well as their relation to the partial sequence and transcription of the h...

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Bibliographic Details
Published in:Mycopathologia (1975) 2008-06, Vol.165 (6), p.355-365
Main Authors: Theodoro, Raquel Cordeiro, de Moraes Gimenes Bosco, Sandra, Araújo, João Pessoa Jr, Candeias, João Manuel Grisi, da Graça Macoris, Severino Assis, Trinca, Luzia Aparecida, Bagagli, Eduardo
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Eukaryotic Microbiology
Fungi
Gene Expression Regulation, Fungal
Genes
Heat
Heat shock proteins
Hot Temperature
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Humans
Life Sciences
Medical Microbiology
Microbial Ecology
Microbiology
Morphogenesis - genetics
Morphogenesis - physiology
Morphology
Mycelium - genetics
Mycelium - growth & development
Paracoccidioides - genetics
Paracoccidioides - isolation & purification
Paracoccidioides - metabolism
Paracoccidioides brasiliensis
Pathogens
Phylogenetics
Plant Sciences
Transcription, Genetic
Virulence
Virulence - genetics
Yeast
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Summary:The mycelia-to-yeast (M-Y) transition, thermal tolerance and virulence profiles were evaluated for nine isolates of Paracoccidioides brasiliensis, including samples from two of the three recently discovered cryptic species, as well as their relation to the partial sequence and transcription of the hsp70 gene. The isolates Bt84 and T10 (from PS2 species) took more time to convert to yeast form and presented elongated yeast cells at 36°C. Arthroconidia production was also observed during the M-Y transition for some isolates. Our data confirm that the hsp70 transcription may be associated with thermal tolerance, but this does not seem to be directly related to high virulence profiles. The partial sequencing of this gene allowed the separation of our isolates into two clusters that correspond to the two sympatric cryptic species occurring in an area hyperendemic for PCM (Botucatu, SP, Brazil).
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-008-9091-6