Identification of KLF13 and KLF14 (SP6), Novel Members of the SP/XKLF Transcription Factor Family

• Published in: Genomics (San Diego, Calif.) Calif.), 2000-11, Vol.70 (1), p.93-101
• Main Authors: Scohy, Sophie, Gabant, Philippe, Van Reeth, Thierry, Hertveldt, Valérie, Drèze, Pierre-Luc, Van Vooren, Pascale, Rivière, Michèle, Szpirer, Josiane, Szpirer, Claude
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cell Cycle Proteins - genetics; chromosome 10; chromosome 15; chromosome 17; chromosome 2; chromosome 6; Chromosome Mapping; Chromosomes, Human, Pair 15; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Humans; KLF11 gene; Klf13 gene; KLF13 protein; Klf14 gene; KLF14 protein; Kruppel-Like Transcription Factors; Mice; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Multigene Family; Rats; Repressor Proteins - genetics; RFLAT-1 protein; Sequence Homology, Amino Acid; SP protein; Sp Transcription Factors; SP6 gene; Trans-Activators - classification; Trans-Activators - genetics; Transcription Factors - classification; Transcription Factors - genetics; Transcription. Transcription factor. Splicing. Rna processing; XKLF protein; Zinc Fingers
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.2000.6362

Using the sequence of the SP1 zinc-finger DNA-binding domain as a probe to screen a mouse EST database, we identified two novel members of the SP/XKLF transcription factor family, KLF13 and KLF14. The mouse Klf13 cDNA (1310 bp in length) contains a single open reading frame of 288 amino acids with a DNA-binding domain closely related to that of the human RFLAT-1 protein and a putative transactivator N-terminal domain rich in proline and alanine residues. The mouse Klf13 gene seems to be the homologue of the human RFLAT1 gene. The mouse Klf14 sequence is homologous to a human genomic sequence from chromosome 17 that is believed to code for a protein with three zinc fingers at the end of its C-terminal domain. Using reverse transcription–polymerase chain reaction, we showed ubiquitous expression of Klf13 and Klf14 in adult mice. A third member of this family was also identified in a human EST database; this sequence was found to be identical to KLF11 (TIEG2), recently identified by Cook et al. (1998, J. Biol. Chem. 273: 25929–25936). The corresponding mouse cDNA was isolated and sequenced. The three genes were localized in the human and the rat: chromosomes 15 (human KLF13), 17q21.3–q22 (human KLF14; HGMW-approved symbol SP6), and 2p25 (human KLF11) and chromosomes 1q31–q32 (rat Klf13), 10q31–q32.1 (rat Klf14) (SP6), and 6q16–q21 (rat Klf11).