Cytochrome P450 3A, NADPH cytochrome P450 reductase and cytochrome b5 in the upper airways in horse

Gene and protein expression as well as catalytic activity of cytochrome P450 (CYP) 3A were studied in the nasal olfactory and respiratory mucosa and the tracheal mucosa of the horse. We also examined the activity of NADPH cytochrome P450 reductase (NADPH P450 reductase), the amount of cytochrome b5...

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Bibliographic Details
Published in:Research in veterinary science 2008-08, Vol.85 (1), p.80-85
Main Authors: Tyden, E, Olsen, L, Tallkvist, J, Tjälve, H, Larsson, P
Format: Article
Language:English
Subjects:
Animals
cytochrome b
cytochrome b5
cytochrome P-450
Cytochrome P-450 CYP3A - genetics
Cytochrome P-450 CYP3A - metabolism
Cytochromes b5 - genetics
Cytochromes b5 - metabolism
digestive tract mucosa
enzyme activity
Female
gene expression
Gene Expression Regulation - physiology
horses
Horses - metabolism
liver
Liver - metabolism
Male
NADPH-cytochrome-c2 reductase
NADPH-Ferrihemoprotein Reductase - genetics
NADPH-Ferrihemoprotein Reductase - metabolism
Nose - metabolism
protein synthesis
Respiratory Mucosa - metabolism
respiratory system
Trachea - metabolism
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Summary:Gene and protein expression as well as catalytic activity of cytochrome P450 (CYP) 3A were studied in the nasal olfactory and respiratory mucosa and the tracheal mucosa of the horse. We also examined the activity of NADPH cytochrome P450 reductase (NADPH P450 reductase), the amount of cytochrome b5 and the total CYP content in these tissues. Comparative values for the above were obtained using liver as a control. The CYP3A related catalytic activity in the tissues of the upper airways was considerably higher than in the liver. The CYP3A gene and protein expression, on the other hand, was higher in the liver than in the upper airway tissues. Thus, the pattern of CYP3A metabolic activity does not correlate with the CYP3A gene and protein expression. Our results showed that the activity of NADPH P450 reductase and the level of cytochrome b5 in the relation to the gene and protein expression of CYP3A were higher in the tissues of the upper airways than in the liver. It is concluded that CYP3A related metabolism in horse is not solely dependent on the expression of the enzyme but also on adequate levels of NADPH P450 reductase and cytochrome b5.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2007.09.012