Effects of calcium ion incorporation on osteoblast gene expression in MC3T3-E1 cells cultured on microstructured titanium surfaces

The surface characteristics of a calcium ion (Ca)‐incorporated titanium (Ti) surface, produced by hydrothermal treatment using an alkaline Ca‐containing solution, and its effects on osteoblastic differentiation were investigated. MC3T3‐E1 pre‐osteoblastic cells were cultured on machined or grit‐blas...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2008-07, Vol.86A (1), p.117-126
Main Authors: Park, Jin-Woo, Suh, Jo-Young, Chung, Hyun-Ju
Format: Article
Language:English
Subjects:
3T3 Cells
Animals
Biocompatible Materials - chemistry
Calcium - chemistry
Cell Culture Techniques - instrumentation
Cell Culture Techniques - methods
Cell Differentiation
differentiation
Ions
Materials Testing
Mice
osteoblast
Osteoblasts - metabolism
Osteocalcin - metabolism
Osteopontin - metabolism
surface calcium chemistry
Surface Properties
surface topography
Tetrazolium Salts - pharmacology
Thiazoles - pharmacology
Titanium - chemistry
titanium implant
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Summary:The surface characteristics of a calcium ion (Ca)‐incorporated titanium (Ti) surface, produced by hydrothermal treatment using an alkaline Ca‐containing solution, and its effects on osteoblastic differentiation were investigated. MC3T3‐E1 pre‐osteoblastic cells were cultured on machined or grit‐blasted Ti surfaces with and without Ca incorporation. The MTT assay was used to determine cell proliferation, and real‐time PCR was used for quantitative analysis of osteoblastic gene expression. Hydrothermal treatment with a Ca‐containing solution produced a crystalline CaTiO3 nanostructure of approximately 100 nm in dimension, preserving original micron‐scaled surface topographies and microroughness caused by machining, blasting, or blasting and etching treatments. After immersion in Hank's balanced salt solution, considerable apatite formation was observed on all surfaces of the Ca‐incorporated samples. Significantly more cell proliferation was found on Ca‐incorporated Ti surfaces than on untreated Ti surfaces (p < 0.001). Quantitative real‐time PCR analysis showed notably higher alkaline phosphatase, osteopontin, and osteocalcin mRNA levels in cells grown on Ca‐incorporated blasted surfaces than on other surfaces at an early time point. Thus, Ca incorporation may have a beneficial effect on osseointegration of microstructured Ti implants by accelerating osteoblast proliferation and differentiation during the early healing phase following implantation. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2008
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.31618