Determination of trace levels of benzodiazepine in urine using capillary electrochromatography-time of flight mass spectrometry

This paper details a method for the separation and determination of ten benzodiazepines in urine using CEC-MS(TOF) and an hexyl acrylate-based porous monolith. The TOF mass spectrometer provides an exact mass of protonated benzodiazepines to three decimal places (1-6 ppm). This high selectivity alon...

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Bibliographic Details
Published in:Electrophoresis 2008-05, Vol.29 (10), p.2182-2192
Main Authors: Blas, Maximilien, McCord, Bruce R
Format: Article
Language:English
Subjects:
Acrylates
Anti-Anxiety Agents - chemistry
Anti-Anxiety Agents - urine
Benzodiazepine
Benzodiazepines - chemistry
Benzodiazepines - urine
Capillary Electrochromatography - instrumentation
Capillary Electrochromatography - methods
Capillary Electrochromatography - statistics & numerical data
Electrochromatography
Humans
Mass spectrometry
Microscopy, Electron, Scanning
Monolith
Spectrometry, Mass, Electrospray Ionization - methods
Spectrometry, Mass, Electrospray Ionization - statistics & numerical data
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Summary:This paper details a method for the separation and determination of ten benzodiazepines in urine using CEC-MS(TOF) and an hexyl acrylate-based porous monolith. The TOF mass spectrometer provides an exact mass of protonated benzodiazepines to three decimal places (1-6 ppm). This high selectivity along with the CEC separation, provides an effective method for the identification of benzodiazepines. Linearity is satisfactory for all compounds in the concentration range of 25-500 ng/mL for lorazepam and 12.5-500 ng/mL for the others. The RSDs are between 1.4-2.3% for retention times and 1.1-9.2% for relative areas. Using the monolithic stationary phase, a preconcentration step is achievable and permits an 75-140-fold improvement in sensitivity. This strategy permits the quantification of these drugs down to 1 ng/mL in urine. This method was used for the analysis of benzodiazepines in spiked urine samples.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.200700422