Quantitative Analysis of Ribonucleoside Triphosphates in Human Lymphoid Cells by Micellar Electrokinetic Capillary Chromatography

• Published in: Annals of clinical biochemistry 1999-09, Vol.36 (5), p.636-641
• Main Authors: Bolzonella, C, Gusella, M, Bononi, A, Loregian, A, Crepaldi, G, Toso, S, Palù, G, Ferrazzi, E
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Chromatography, High Pressure Liquid; Chromatography, Micellar Electrokinetic Capillary; Hematology; Humans; Investigative techniques, diagnostic techniques (general aspects); Leukemia, Lymphocytic, Chronic, B-Cell - metabolism; Lymphoma - metabolism; Medical sciences; Nucleotides - metabolism; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Reproducibility of Results; Skin Neoplasms - metabolism
• ISSN: 0004-5632; 1758-1001
• DOI: 10.1177/000456329903600512

Micellar electrokinetic capillary chromatography (MECC) was applied to develop an analytical method for quantitation of ribonucleoside triphosphates (rNTPs) in human lymphoid cells obtained from patients with B-chronic lymphocytic leukaemia (B-CLL) and cutaneous lymphomas. The results of this analysis showed a significant depression of intracellular rNTPs in patients with B-CLL, compared with rNTPs of healthy controls. These data are in agreement with other studies in which rNTP separations were performed with traditional high-performance liquid chromatography. MECC has proved to be a useful tool for intracellular rNTPs determination, revealing possible new applications in the study of the metabolic state of human cells. In addition, this method can be useful in monitoring the effect of many drugs (antiviral, antineoplastic) which interfere with nucleotide metabolism.