Characterization of the unconventional myosin VIII in plant cells and its localization at the post-cytokinetic cell wall

Myosins are a large superfamily of motor proteins which, in association with actin, are involved in intracellular motile processes. In addition to the conventional myosins involved in muscle contractility, there is, in animal cells, a wide range of unconventional myosins implicated in membrane-assoc...

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Published in:The Plant journal : for cell and molecular biology 1999-09, Vol.19 (5), p.555-567
Main Authors: Reichelt, S, Knight, A.E, Hodge, T.P, Baluska, F, Samaj, J, Volkmann, D, Kendrick-Jones, J
Format: Article
Language:English
Subjects:
actin
Arabidopsis - enzymology
Arabidopsis thaliana
Biological and medical sciences
Cell Communication
Cell kinetics
Cell physiology
cell wall components
cell walls
cytoskeleton
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
immunocytochemistry
Ion Channel Gating
Isoenzymes - physiology
Lepidium sativum
Microscopy, Confocal
Mitosis
motility
myosin
Myosins - physiology
Plant physiology and development
Plant Roots - enzymology
plasmodesmata
roots
Zea mays
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Summary:Myosins are a large superfamily of motor proteins which, in association with actin, are involved in intracellular motile processes. In addition to the conventional myosins involved in muscle contractility, there is, in animal cells, a wide range of unconventional myosins implicated in membrane-associated processes, such as vesicle transport and membrane dynamics. In plant cells, however, very little is known about myosins. We have raised an antibody to the recombinant tail region of Arabidopsis thaliana myosin 1 (a class VIII myosin) and used it in immunofluorescence and EM studies on root cells from cress and maize. The plant myosin VIII is found to be concentrated at newly formed cross walls at the stage in which the phragmoplast cytoskeleton has depolymerized and the new cell plate is beginning to mature. These walls are rich in plasmodesmata and we show that they are the regions where the longitudinal actin cables appear to attach. Myosin VIII appears to be localized in these plasmodesmata and we suggest that this protein is involved in maturation of the cell plate and the re-establishment of cytoplasmic actin cables at sites of intercellular communication.
ISSN:0960-7412
1365-313X
DOI:10.1046/j.1365-313x.1999.00553.x