Development of a polymerase chain reaction to detect Vietnamese isolates of duck virus enteritis

A polymerase chain reaction (PCR) method for the detection of duck virus enteritis (DVE) virus in tissues of infected and affected ducks, and in cell culture was developed. This required us to obtain specific nucleotide sequence information as we could not find any specific data about the genome of...

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Bibliographic Details
Published in:Veterinary microbiology 1999-08, Vol.68 (1), p.149-156
Main Authors: Pritchard, L.I., Morrissy, C., Van Phuc, Kim, Daniels, P.W., Westbury, H.A.
Format: Article
Language:English
Subjects:
Anatid herpesvirus
Animal viral diseases
Animals
Biological and medical sciences
CANARD
Cells, Cultured
Chick Embryo
Deoxyribonuclease BamHI - chemistry
DIAGNOSIS
DIAGNOSTIC
DIAGNOSTICO
DNA Primers - chemistry
DNA, Viral - chemistry
DNA, Viral - isolation & purification
Duck plague
Duck viral enteritis
Duck virus enteritis virus
DUCKS
Electrophoresis, Agar Gel - veterinary
ENTERITE
ENTERITIS
Enteritis - diagnosis
Enteritis - genetics
Enteritis - veterinary
Esophagus - virology
Fundamental and applied biological sciences. Psychology
Gene Library
Herpesviridae Infections - diagnosis
Herpesviridae Infections - genetics
Herpesviridae Infections - veterinary
Herpesvirus 1, Gallid - genetics
Herpesvirus 1, Gallid - isolation & purification
HERPETOVIRIDAE
Infectious diseases
Liver - virology
Medical sciences
Microbiology
NUCLEOTIDE SEQUENCE
PATO
PCR
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Poultry Diseases - diagnosis
Poultry Diseases - genetics
SECUENCIA NUCLEOTIDICA
Sequence Analysis, DNA
SEQUENCE NUCLEOTIDIQUE
Techniques used in virology
Vietnam
Viral diseases
Virology
VIRUS
VIRUSES
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Summary:A polymerase chain reaction (PCR) method for the detection of duck virus enteritis (DVE) virus in tissues of infected and affected ducks, and in cell culture was developed. This required us to obtain specific nucleotide sequence information as we could not find any specific data about the genome of the virus. We found the assay to be highly effective in detecting the virus under experimental conditions and to be easily transferred to laboratories in Vietnam where it is being used in studies on the epidemiology of the disease. We have applied this simple and rapid diagnostic method to the detection of DVE isolates grown in cell culture and tissues from infected birds. The assay was also able to differentiate DVE from other avian herpesviruses, such as Marek’s disease, infectious laryngotracheitis virus and goose herpesvirus.
ISSN:0378-1135
1873-2542
DOI:10.1016/S0378-1135(99)00071-1