Effect of the knobbed acrosome defect in bovine sperm on IVF and embryo production

• Published in: Theriogenology 2000-10, Vol.54 (6), p.921-934
• Main Authors: Thundathil, J., Meyer, R., Palasz, A.T., Barth, A.D., Mapletoft, R.J.
• Format: Article
• Language: English
• Subjects: Acrosome - physiology; Animals; bull; Cattle - embryology; Cattle - physiology; Coloring Agents - chemistry; Female; fertility; Fertilization in Vitro - veterinary; IVF; knobbed acrosome; Male; Microscopy, Phase-Contrast - veterinary; Oocytes - physiology; Oxazines - chemistry; Random Allocation; semen; sperm morphology; Sperm-Ovum Interactions - physiology; Spermatozoa - abnormalities; Statistics, Nonparametric
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/S0093-691X(00)00402-7

An IVF and culture system was used to determine the effect of the knobbed acrosome defect in bovine spermatozoa on fertilization and early embryonic development. Three bulls affected with knobbed acrosomes were identified as K + (flattened acrosome), K 2+ (indented acrosome) or K 3+ (deep indentation of the acrosome) based on the predominant type of acrosomal aberration present in sperm of the respective bulls. After swim-up, all semen traits, except for acrosome morphology, were similar between bulls with varying degrees of the knobbed acrosome defect and a control bull, C. The mean number of spermatozoa bound to the zona pellucida was lower (P< 0.05) for the bulls with the knobbed acrosome defects (40.3 ± 2.3, 29.5 ± 1.6, 14.6 ± 1.3, respectively, for Bulls K +, K 2+ and K 3+) than for Bull C (52.3 ± 2.3). The percentages of zonae pellucidae penetrated by spermatozoa from Bulls K + (51.2%), K 2+ (49.5%) and K 3+ (37.1%) were lower than that of Bull C (84.5%). No sperm with knobbed acrosome defects were found to have penetrated the zona pellucida. Fertilization rates for bulls with the knobbed acrosome defect, K + (63.0%), K 2+ (62.7%) and K 3+ (22.6%), were significantly lower than that of the control bull (82.8%). Percentages of cleaved embryos, morulae and blastocysts produced were also lower for the bulls with knobbed acrosomes than that of the control bull. Results indicate that sperm with the knobbed acrosome defect had a reduced ability to bind to the zona pellucida, depending upon the severity of the defect, and that these aberrant spermatozoa did not penetrate the zona pellucida. The apparently normal spermatozoa coexisting in the inseminate of bulls with a high percentage of knobbed spermatozoa were also functionally deficient; oocytes penetrated by these spermatozoa had a reduced potential for fertilization, and resulting zygotes had a reduced ability for cleavage and embryonic development to the blastocyst stage. The results of the present study do not support the hypotheses that the knobbed acrosome defect is compensable.