A type 2A protein phosphatase gene from Aspergillus nidulans is involved in hyphal morphogenesis

A PCR-based approach, using degenerate oligonucleotide primers, was used to isolate fragments of two genes encoding type 2A protein phosphatases from the filamentous fungus, Aspergillus nidulans. The complete genomic sequence of one of these genes, pphA, was isolated and characterised. The pphA gene...

Full description

Saved in:
Bibliographic Details
Published in:Current genetics 2001-02, Vol.39 (1), p.25-34
Main Authors: Kosmidou, E, Lunness, P, Doonan, J H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aspergillus nidulans
Aspergillus nidulans - genetics
Aspergillus nidulans - growth & development
Base Sequence
Bisbenzimidazole - pharmacology
Crosses, Genetic
DNA, Fungal
Genes, Fungal
Genotype
Molecular Sequence Data
Morphogenesis - genetics
Phosphoprotein Phosphatases - genetics
Phosphoprotein Phosphatases - physiology
Polymerase Chain Reaction
pphA gene
Protein Phosphatase 2
protein phosphatase 2A
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A PCR-based approach, using degenerate oligonucleotide primers, was used to isolate fragments of two genes encoding type 2A protein phosphatases from the filamentous fungus, Aspergillus nidulans. The complete genomic sequence of one of these genes, pphA, was isolated and characterised. The pphA gene was predicted to encode a 329-residue protein which is about 85% identical to mammalian protein phosphatase 2A. Ectopic expression of the wild-type pphA+ gene slightly inhibited growth in some transformants; but a mutant form of pphA, in which R259 was mutated to Q, led to slow growth, delayed germ tube emergence and mitotic defects at low temperature. These results indicate that the pphA+ gene plays an important role in hyphal growth.
ISSN:0172-8083
1432-0983
DOI:10.1007/s002940000177